Transformant for screening of inhibitors for human...

Details Transformant for screening of inhibitors for human... Transformant for screening of inhibitors for human...

2006-04-25

2006-04-25

Parkin, Jeffrey S. (Department: 1648)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

C424S188100

Reexamination Certificate

active

07033596

ABSTRACT:
A microorganism cotransformed with a gene expressing HIV nucleocapsid protein and a plasmid vector containing HIV ψ gene and β-galactosidase reporter gene, and a method for screening HIV inhibitors employing the transformant. The invented method comprising the steps of culturing the transformant, treating it with putative compounds or compositions of HIV inhibitors, and measuring the degree of change in β-galactosidase expression in the culture, can be practically applied in screening HIV packaging inhibitors by which the interaction between HIV nucleocapsid and HIV ψ sequence is blocked.

REFERENCES:
Bacharach, E., and S. P. Goff, 1998, “Binding of the Human Immunodeficiency Virus Type 1 Gag Protein to the Viral RNA Encapsidation Signal in the Yeast Three-Hybrid System”, J. Virol. 72(8):6944-6949.
Strair, R. K., et al., 1993. “Recombinant Retroviral Systems for the Analysis of Drugs Resistant HIV”, Nuc. Acids Res. 21(20):4836-4842.
“HIV Nucleocapsid Protein, Expression inEscherichia coli, Purification, and Characterization,” J.C. You et al., J. Biol. Chem.,Aug. 5, 1993, 268(22): pp 16519-16527.
“Recombinant retroviral systems for the analysis of drug resistant HIV,” R.K. Stair et al., Nucieic Acids Res. Oct. 1993, 21(20) pp 4836-4842.
“Beta-Galactosidase containing a human immunodeficiency virus protease cleavage site is cleaved and inactivated by human immunodeficiency virus protease,” E.Z. Baum et al.,Proc Natl Acad Sci USA, Dec. 1990, 87(24), pp 10023-10027.

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