Transformant and process for producing polyester by using...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Fungi

Reexamination Certificate

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C435S193000, C435S232000

Reexamination Certificate

active

07083972

ABSTRACT:
The present invention relates to a gene coding for a copolyester-synthesizing enzyme, a microorganism which utilizes the gene for the fermentative synthesis of a polyester, and a method of producing a polyester with the aid of the microorganism. More particularly, the present invention relates to a gene which functions in a host organism and, by an enzyme, synthesizes a plastic-like polymer degradable under the action of microorganisms in the natural environment (the soil, river or sea). The present invention also, more particularly, relates to a transformant derived from the host organism by transformation with the gene and having an improved ability to fermentatively synthesize a plastic-like polymer, and a method of producing a copolyester with the aid of the transformant.

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patent: 10-108682 (1998-04-01), None
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Timothy A. Leaf, et al., “Saccharomyces cerevisiaeexpressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate”,Microbiology142: pp. 1169-1180 (1996).
Toshiaki Fukui, et al., “Co-expression of polyhydroxyalkanoate synthase and (R)-enoyl-CoA hydratase genes ofAeromonas caviaeestablishes copolyester biosynthesis pathway inEscherichia coli”, FEMS Microbiology Letters170 pp. 69-75 (1999).
T. Fukui, et al., “Cloning and Analysis of the Poly(3-Hydroxybutyrate-co-3-Hydroxyhexanoate) Biosynthesis Genes ofAeromonas caviae,”J. Bacteriology, vol. 179, No. 15, Aug. 1997, pp. 4821-4830.
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