Transfer vector

Details Transfer vector Transfer vector

1987-03-09

1989-12-05

Warren, Charles F.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4352523, 43525233, 435320, 536 27, 935 31, 935 39, 935 72, 935 73, C12N 1500, C12R 141, C12R 1185, C07H 1512

Patent

active

048852481

ABSTRACT:
A recombinant DNA vector is provided as a universal transcription vector having a replication origin and selectable marker, a promoter and a transcription initiation site comprising a first transcribed nucleotide, wherein a restriction site is provided immediately adjacent to and upstream from the transcription initiation site so as to separate transcribed from untranscribed nucleotides. A second restriction site may also be positioned downstream from the said restriction site. Precise control of initiation and termination of transcription is attained by this invention. Such control is important in assuring the effectiveness of transcribed RNA viral vectors.
A high fidelity in vitro RNA transcription method is also provided utilizing vectors constructed from the universal transcription vector, or other vectors producing transcripts having no more than one extra 5' base. This method is capable of producing functional RNA transcripts, preferably comprising infectious viral sequences.

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Soberon et al., (1982), Promoters: Structure and Function, in Rodriguez et al., (eds.), Praeger Publishers, New York, N.Y., p. 407.
Pouwels et al., (1985), in Cloning Vectors: A Laboratory Manual, Elsevier, Amsterdam, The Netherlands, p. 1-B-iv-4.
Kessler et al., (1986), Gene 47: 1-153.
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Dunn et al., (1983), J. Mol. Biol. 166:477-535.
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