Transfer vector

Details Transfer vector Transfer vector

1989-10-03

1992-12-22

Fox, David T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 691, 4351723, 4352351, 4353171, 536 27, 935 4, 935 17, 935 18, 935 21, 935 41, C12P 1934, C12N 1500, C12N 700, C07H 1512

Patent

active

051734109

ABSTRACT:
A recombinant DNA vector is provided as a universal transcription vector having a replication origin and selectable marker, a promoter and a transcription initiation site comprising a first transcribed nucleotide, wherein a restriction site is provided immediately adjacent to and upstream from the transcription initiation site so as to seprate transcribed from untranscribed nucleotides. A second restriction site may also be positioned downstream from the said restriction site. Precise control of initiation and termination of transcription is attained by this invention. Such control is important in assuring the effectiveness of transcribed RNA viral vectors.
A high fidelity in vitro RNA transcription method is also provided utilizing vectors constructed from the universal transcription vector, or other vectors producing transcripts having no more than one extra 5' base. This method is capable of producing functional RNA transcripts, preferably comprising infectious viral sequences.

REFERENCES:
patent: 4766072 (1988-08-01), Jendrisak et al.
patent: 4885248 (1989-12-01), Ahlquist
Kang et al. 1987, Nucleic Acids Res. 15(5): 2279-2294.
Contreras et al. 1982, Nucleic Acids Research 10(20): 6353-6362.
Myers et al. 1977, Proc. Natl. Acad. Sci. USA 74(7): 2840-2843.
Melton et al. 1984, Nucl. Acids Res. 12(18): 7035-7056.
Kennell, D. 1985, pp. 101, 110, 137-138 In: Maximizing gene expression, Reznekoff et al., eds., Butterworth Press: Boston.

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