Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...
Patent
1995-02-17
1997-04-15
Marx, Irene
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing heterocyclic carbon compound having only o, n, s,...
435 41, 435420, 549510, C12P 1702, C12P 100
Patent
active
056208758
ABSTRACT:
Taxol, an antineoplastic drug, has shown cytotoxic activity against numerous malignant tumors including: breast cancer, colon cancer, ovarian cancer, and skin cancer. Taxol is found in many species of yew including: slow-growing Taxus brevifolia and faster-growing Taxus media X Hicksii. Yew tree cuttings were cultured and the medium was extracted weekly, bi-weekly, and tri-weekly over a period of nine weeks. Taxol and other taxanes were separated through a multi-step procedure using hexane extractions, C-18 Sep Pak cartridges, and high performance liquid chromatography (HPLC). The concentration of the taxanes was then calculated. Taxol and other taxanes can continue to be transferred into a culture medium and extracted over a nine-week period. There is a higher yield when extracted weekly.
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Franzen Alyssa T.
Hoffman Angela
Voelker Courtney C. J.
Marx Irene
University of Portland
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