Transfer of taxol from yew tree cuttings into a culture medium o

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing heterocyclic carbon compound having only o – n – s,...

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435 41, 435420, 549510, C12P 1702, C12P 100

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056208758

ABSTRACT:
Taxol, an antineoplastic drug, has shown cytotoxic activity against numerous malignant tumors including: breast cancer, colon cancer, ovarian cancer, and skin cancer. Taxol is found in many species of yew including: slow-growing Taxus brevifolia and faster-growing Taxus media X Hicksii. Yew tree cuttings were cultured and the medium was extracted weekly, bi-weekly, and tri-weekly over a period of nine weeks. Taxol and other taxanes were separated through a multi-step procedure using hexane extractions, C-18 Sep Pak cartridges, and high performance liquid chromatography (HPLC). The concentration of the taxanes was then calculated. Taxol and other taxanes can continue to be transferred into a culture medium and extracted over a nine-week period. There is a higher yield when extracted weekly.

REFERENCES:
Arbuck, Susan G. et al., Clinical Development of Taxol, 1993a, Journal of the National Cancer Institute Monographs, 15:11-24.
Arbuck, Susan G. et al., A Reassessment of Cardiac Toxicity Associated with Taxol, Journal of the National Cancer Institute Monographs, 15:117-130, 1993.
Coughlan, A., Yew tree yields anticancer culture, New Scientist, 9 Jan. 1993, p. 21.
Fett-Neto and DiCosmo, Distribution and Amounts of Taxol in Different Shoot Parts of Taxus cuspidata, Planta Med. 58:464-466, 1992.
Guenard, D., et al., Taxol and Taxotere: Discovery, Chemistry, and Structure-Activity Relationships, Acc. Chem. Res., 1993, 26, 160-67.
Hei and Hall, Taxol, Radiation, and Oncogenic Transformation, Cancer Research 53:1368-72, Mar. 15, 1993.
Hoffman and Franzen, Taxane Production by Yew Tree Cuttings, Proceedings of the Oregon Academy of Science, vol. XXX, 20:22.
Joyce, C., Taxol: search for a cancer drug, BioScience 43 (3):133-36 (1993).
Millipore Waters Chromatography, 1993-94. The Waters Chromatography Handbook, Milford, Massachusetts; Millipore Corporation. P. 20-22, 30-31.
Richeimer, et al., High-Performance Liquid Chromatographic Assay of Taxol, Anal. Chem., 64:2323-36, 1995.
Sigma Chemical Company Catalog, 1991-92; Plant Cell Culture, St. Louis, p. 53.
Taxol Gains Quick FDA Approval, Science 259:181 (8 Jan. 1993).
U.S. Department of Health and Human Services, 1990, NCI Investigational Drugs: Pharmaceutical Data, 151-53.
Witherup et al., High Performance Liquid Chromatographic Separation of Taxol and Related Compounds From Taxus brevifolia, Journal of Liquid Chromatoraphy 12:11, 2117-2132 (1989).
Wickremesinhe et al, J of Liquid Chromatography, vol. 16, pp. 3263-3274(1993).
Strobel, et al., Plant Science, vol. 84, pp. 65-74, 1992.

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