Transfer of macromolecules from a chromatographic substrate to a

Details Transfer of macromolecules from a chromatographic substrate to a Transfer of macromolecules from a chromatographic substrate to a

1985-01-04

1986-07-22

Adee, John

Liquid purification or separation

Processes

Liquid/liquid solvent or colloidal extraction or diffusing...

210645, 2101983, B01D 1508

Patent

active

046018280

ABSTRACT:
The method of transfer of macromolecules such as nucleic acid and proteins from a chromatographic substrate to an immobilizing matrix uses as the immobilizing matrix, a charge modified microporous membrane comprising an organic microporous membrane having a charge modifying amount of a cationic charge modifying agent bonded to substantially all of the wetted surfaces of said membrane. The charge modified microporous membrane can also be a reinforced microporous membrane, preferably a porous reinforcing web impregnated with a polymeric microporous membrane. A nucleic acid or protein blotting product comprising a chromatographic matrix having the charge modified microporous membrane on a surface thereof is also provided.

REFERENCES:
patent: 3875044 (1975-04-01), Renn et al.
patent: 3878100 (1975-04-01), Bixler
patent: 4243507 (1981-01-01), Martin et al.
patent: 4297198 (1981-10-01), Ohashi et al.
patent: 4352884 (1982-10-01), Nakashima et al.
patent: 4415428 (1983-11-01), Nochumson et al.
patent: 4512896 (1985-04-01), Gershoni
Chromatographs and Allied Methods of Mikes, John Wiley & Sons of New York, pp. 663-667, 1979.

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