Transfer and detection of nucleic acids

Details Transfer and detection of nucleic acids Transfer and detection of nucleic acids

1979-07-02

1981-11-24

Wiseman, Thomas G.

Chemistry: physical processes

Physical processes

Crystallization

23230B, 422 56, 424 15, 435 6, 435172, 435805, C12Q 168, G01N 3122, G01N 3316, G01N 3348

Patent

active

043022049

ABSTRACT:
Improvements in the transfer and detection of separated nucleic acids, both RNA and DNA, are provided. For analysis of large DNA, the molecular weight segregated fractions of DNA are depurinated and fragmented to provide fractions having less than about 2 kb as single strands. With both RNA and DNA, the nucleic acid fractions are transferred after resolution to a chemically treated substrate and covalently affixed to the substrate. The resulting nucleotides affixed to the substrate are hybridized with labeled nucleotide probes and a volume exclusion agent, particularly a water soluble ionic polymer.

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patent: 4072574 (1978-02-01), Loeb et al.
patent: 4139346 (1979-02-01), Rabbani
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Wetmur, "Acceleration of DNA Renaturation Rates", Chem. Absts., vol. 84, No. 7, p. 140 (1976), Abs. No. 39846g.
Reiser et al., "Transfer of Small DNA Fragments From Polyacrylamide Gels to Diazobenzyloxymethyl-Paper and Detection by Hybridization With DNA Probes", Biochem. Biophys. Res. Comm., vol. 85, No. 3 (1978), pp. 1104-1112.
Kohne et al., "Room Temperature Method for Increasing the Rate of DNA-Association by Many Thousand Fold: The Phenol Emulsion-Association Technique", Biochem., vol. 16, No. 24 (1977), pp. 5329-5341.

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