Transfection of enteric parasites

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 691, 435 914, 4353201, 435243, 530300, 530350, 536 231, 536 241, C12Q 168, C12N 100, C12N 1585, C12P 1934

Patent

active

058916342

ABSTRACT:
The invention provides methods for expressing foreign genes in enteric protozoa. This transfection system was established using a gene ligated to the 5' and 3' flanking DNA regions of a protein-encoding gene from an enteric protozoa. The present invention also provides such transformed enteric protozoa, vaccines produced therefrom and foreign or altered proteins expressed in the same. The ability to introduce and express genes in amebae will now permit both genetic analysis and modification of the virulence of this organism, which remains a serious threat to world health and will facilitate basic research towards the control of this parasite.

REFERENCES:
Purdy et al. Upstream regulatory elements controlling expression of the Entamoeba hisotlytica lectin. Mol. and Biochem. Parasitol. vol. 78(1-2):91-103, 1996.

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