Transfection of enteric parasites

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351723, 4352523, 4353201, 536 241, 935 22, 935 23, 935 33, 935 52, 935 55, 935 56, 935 66, C12P 2106, C12N 1500, C12N 120, C07H 2104

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056655657

ABSTRACT:
The invention provides methods for expressing foreign genes in enteric protozoa. This transfection system was established using a gene ligated to the 5' and 3' flanking DNA regions of a protein-encoding gene from an enteric protozoa. The present invention also provides such transformed enteric protozoa, vaccines produced therefrom and foreign or altered proteins expressed in the same. The ability to introduce and express genes in amebae will now permit both genetic analysis and modification of the virulence of this organism, which remains a serious threat to world health and will facilitate basic research towards the control of this parasite.

REFERENCES:
Rose Nickel et al., "Transfection and Transient Expression of Chloramphenicol Acetyltransferase Gene in the Protozoan Parasite Entamoeba histolytica", Proc. Natl. Acad. Sci., vol. 91, pp. 7095-7098, Jul. 1994.
Jay E. Purdy et al., "Transient Transfection of the Enteric Parasite Entamoeba histolytica and Experssion of Firefly Luciferase", Proc. Natl. Acad. Sci., vol. 91, pp. 7099-7103, Jul. 1994.
Soldati and Boothroyd, "Transient Transfection and Expression in the Obligate Intracellular Parasite Toxoplasma gondii", Science 260: 349-352 Apr. 1993.
Furfine and Wang, "Transfection of the Giardia iamblia double-stranded RNA virus into Giardia iamblia by electroporation of a single-stranded RNA copy of the viral genome", Mol. Cell. Biol. 10(7): 3659-3663 Jul. 1990.
Biorad Life Science Research Products Catalog, pp. 174-176 1993.
Gauss and Lieber, "DEAE-dextran enhances electroporation of mammalian cells", Nucleic Acids Res. 20(24): 6739-40 Dec. 1992.

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