Electricity: electrical systems and devices – Electrostatic capacitors – Fixed capacitor
Patent
1996-04-10
1998-03-17
Nguyen, Matthew V.
Electricity: electrical systems and devices
Electrostatic capacitors
Fixed capacitor
H01G 438
Patent
active
057294267
ABSTRACT:
The present invention provides apparatus and method for simply and efficiently improving prior art transfection systems. The present invention is a transfection high-voltage controller that provides features for measuring actual load resistance, and compensating for any effects of a limit impedance in series with the load. Additionally, the transfection controller of the present invention measures actual effective capacitance of a storage capacitor, or capacitors, and through use of an expansion module, effectively provides the user with a set of user-selectable capacitors having an effective .+-.10% tolerance when the tolerance of the individual electrolytic capacitors is .+-.20%. The preferred embodiment incorporates two sections. One section of the system handles 200-2500V and another section handles low voltages (50-500V). The transfection controller includes an improved hi-joule switch that operates in a low-voltage, high-current mode with a low dropout voltage (for use with the 500V (maximum) electrolytic capacitors. Further, additional protective circuitry in the form of a crowbar circuit, will not only prevent charging of the storage capacitor unless a safety switch is operated, charge from the capacitor is shunted to ground should the charging operation be interrupted, or if power is removed from the transfection system.
REFERENCES:
patent: 4999565 (1991-03-01), Nilsson
patent: 5077486 (1991-12-01), Marson et al.
Bio-Rad Laboratories
Nguyen Matthew V.
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