Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Parasitic organism or component thereof or substance...
Patent
1996-05-31
1999-11-02
Ketter, James
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Parasitic organism or component thereof or substance...
4242731, 435 6, 435 691, 4352581, 4352584, 435476, 514 44, A61K 39002, C12Q 168, C12P 2102
Patent
active
059765534
ABSTRACT:
A method of transient transfection of obligate intracellular parasites is described. This method is exploited to develop a system for stable transformation utilizing selectable genes. For example, introduction of the chloramphenicol acetyl transferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection results in parasites stably expressing CAT. DNA hybridization analysis indicated that the CAT gene had inserted via homologous recombination.
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Boothroyd John C.
Kim Kami
Soldati Dominique
Fabian Gary R.
Ketter James
Petithory Joanne R.
Sholtz Charles K.
The Board of Trustees of the Leland Stanford Junior University
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