Transfection and genetic manipulations in obligate intracellular

Details Transfection and genetic manipulations in obligate intracellular Transfection and genetic manipulations in obligate intracellular

1993-11-04

1997-07-01

Fleisher, Mindy

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 4351723, 4352581, C12Q 168, C12N 111, C12N 1564, C12P 2102

Patent

active

056437188

ABSTRACT:
A method of transient transfection of obligate intracellular parasites is described. This method is exploited to develop a system for stable transformation utilizing selectable genes. For example, introduction of the chloramphenicol acetyl transferase (CAT) gene fused to Toxoplasma flanking sequences followed by chloramphenicol selection results in parasites stably expressing CAT. DNA hybridization analysis indicated that the CAT gene had inserted via homologous recombination.

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