Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-09-29
1991-05-21
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 435255, 4352402, 435212, 4351723, 435 701, 536 27, 935 60, 935 65, 935 27, 935 33, 935 36, 935 69, 935 70, C12N 1500, C12N 1563, C12N 1579
Patent
active
050174789
ABSTRACT:
This invention relates generally to recombinant DNA techniques and to the expression of mammalian polypeptides in genetically engineered eukaryotic cells. Specifically, the invention relates to preferred plasmid constructs and methods that increase levels of expression of a cloned gene product. These preferred plasmids have selectable and nonselected gene cassettes adjacent to each other and oriented in the opposite direction for transcription. Such an orientation enhances overall levels of expression for the nonselected gene. Further, this invention relates to gene products expressed at these very high levels by the herein described method, and to the eukaryotic cells derived thereby.
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Begley Kathi
Cashion Linda
Colby Wendy
Morser Michael J.
Berlex Laboratories Inc.
Nolan S. L.
Schwartz Richard A.
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