Transesterification method using lipase powder with a particle d

Details Transesterification method using lipase powder with a particle d Transesterification method using lipase powder with a particle d

1994-08-31

1996-01-02

Knode, Marian C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435135, 435198, C12P 764, C12P 762, C12N 920

Patent

active

054807875

ABSTRACT:
The transesterification method is conducted with a powdery lipase wherein the powdery lipase is dispersed in ester-containing starting materials in the presence or absence of an inert organic solvent, and the diameter of at least 90% of the dispersed lipase particles is kept in the range of 1 to 100 .mu.m in the course of the trans-esterification reaction. A reactivity higher than that realized by the conventional transesterification method with a powdery lipase is obtained and the lipase can be easily recovered and reused, without causing a loss of the lipase activity, lowering of the conversion due to the residence of the substrate on the immobilizing carrier and side reactions caused by water introduced into the reaction system by the immobilizing carrier.

REFERENCES:
patent: 5190968 (1993-03-01), Kokusho et al.
patent: 5316927 (1994-05-01), Zaks et al.
Terrades F. et al, J. Am. Chem. Soc. 115:390-396 (1993).
Klibanov A. M., TIBS 14:141-144 (1989).

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