Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2005-09-27
2005-09-27
McGarry, Sean (Department: 1635)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S006120, C435S375000, C435S336000, C435S384000, C536S024500
Reexamination Certificate
active
06949380
ABSTRACT:
Disclosed is a method of transdifferentiating an epidermal basal cell into a cell having one or more morphological, physiological and/or immunological features of a neural progenitor, neuronal, or glial cell by culturing a proliferating epidermal basal cell population derived from the skin of a mammalian subject; exposing the epidermal basal cell(s) to an antagonist of bone morphogenetic protein (BMP), such as fetuin, noggin, chordin, gremlin, or follistatin; and growing the cell(s) in the presence of at least one antisense oligonucleotide comprising a segment of a human MSX1 gene and/or a segment of a human HES1 gene, or homologous non-human counterpart of either of these. Also disclosed is a transdifferentiated cell of epidermal origin and cell cultures derived therefrom. In addition, methods of using the inventive transdifferentiated cell(s) and cell cultures to identify a novel nerve growth factor or to screen a potential chemotherapeutic agent by detecting the presence or absence of an effect, in vitro, on a morphological, physiological and/or molecular biological property of the transdifferentiated cell(s) are described, as is a method of using the transdifferentiated cell(s) and cell cultures to screen a potential chemotherapeutic agent to treat a nervous system disorder of genetic origin. A kit useful for practicing the methods is disclosed
REFERENCES:
patent: 4560655 (1985-12-01), Baker
patent: 5041538 (1991-08-01), Ling et al.
patent: 5143842 (1992-09-01), Ham et al.
patent: 5182375 (1993-01-01), Ling et al.
patent: 5324656 (1994-06-01), Ham et al.
patent: 5411883 (1995-05-01), Boss et al.
patent: 5589376 (1996-12-01), Anderson et al.
patent: 5670481 (1997-09-01), Harland et al.
patent: 5679783 (1997-10-01), De Robertis et al.
patent: 5753506 (1998-05-01), Johe
patent: 5821124 (1998-10-01), Valenzuela et al.
patent: 5843775 (1998-12-01), Valenzuela et al.
patent: 5846770 (1998-12-01), LaVallie et al.
patent: 5902785 (1999-05-01), Hattersley et al.
patent: 5986056 (1999-11-01), La Vallie et al.
patent: WO 97/11095 (1997-03-01), None
Branch, TIBS 23, pp. 45-50, Feb. 1998.
Flanagan et al., Nature Biotech. 17: 48-52, Jan. 1999.
Chang et al., Development (Eng), 126(15) p. 3347-57, Aug. 1999.
Adam, E. et al.,Neuraminic acid specific lectins as markers of early cortical plate neurons, Int. J. Dev. Neurosci,11(4):451-60 (Aug. 1993) Abstract Only.
Bellefroid, Eric J. et al.,X-MyT1, a Xenopus C2HC-Type Zinc Finger Protein with a Regulatory Function in Neuronal Differentiation, Cell,vol. 87, 1191-1202, Dec. 1996.
Brown, T.J., et al.Characterization of 11 beta-methoxy-16 alpha-[125l] iodoestradial binding: neuronal localization of estrogen-binding sites in the developing rat brain. Endocrinology,124(5):2074-88 (May 1989) Abstract Only.
Cassiman, D., et al.,Synaptophysin: A Novel Marker for Human and Rat Hepatic Stellate Cells, Am. J. Pathol,155(6):1831-1839 (Dec. 1999) Abstract Only.
Demetriou M., et al.,Fetuin/alpha 2-HS glycoprotein is a transforming growth factor-beta type II receptor mimic and cytokine antagonist, J. Biol. Chem. ,271(22):12755-61 (May 1996) Abstract Only.
Dziegielewska K.M., et al.,Synthesis of the foetal protein fetuin by early developing neurons in the immature neocortex, J. Neurocytol,22(4):266-72 (Apr. 1993). Abstract Only.
Fann, M.J., et al.,Depolarization differentially regulates the effects of bone morphogenetic protein(BMP)-2, BMP-6, and activin A on sympathetic neuronal phenotype, J. Neurochem,63(6):2074-9 (Dec. 1994) Abstract Only.
Furuta, Y., et al.,Bone morphogenetic proteins(BMPs)as regulators of dorsal forebrain development, Development,124(11):2203-12 (Jun. 1997) Abstract Only.
Guillemot, F., et al.,Dynamic expression of the murine Achaete-Scute homologue Mash-1 in the developing nervous system, Mech. Dev.,42(3):171-85 (Aug. 1993) Abstract Only.
Hattori, A., et al.,Bone morphogenetic protein-2 promotes survival and differentiation of striatal GABAergic neurons in the absence of glial cell proliferation, J. Neorochem,72(6):2264-71 (Jun. 1999) Abstract Only.
Hirota Y., et al.,Musashi and seven in absentia downregulate tramtrack through distinct mechanisms in drosophila eye development, Mech. Dev.,87(1-2):93-101 (Sep. 1999) Abstract Only.
Iantosca, M.R., et al.,Bone morphogenetic proteins-2 and -4 attenuate apoptosis in a cerebellar primitive neuroectodermal tumor cell line, J. Neurosci Res.,56(3):248-58 (May 1999) Abstract Only.
Ishibashi, M., et al.,Targeted disruption of mammalian hairy and Enhancer of split homolog-1(HES-1)leads to up-regulation of neural helix-loop-helix factors, premature neurogenesis, and severe neural tube defects, Genes&Development,9:3136-3148 (1995).
Ishibashi, M., et al.,Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system, The EMBO Journal,vol. 13, No. 8, pp. 1799-1805 (1994).
Ishizeki, K., et al.,Sequential synthesis of cartilage and bone marker proteins during transdifferentiation of mouse Meckel's cartilage chondrocytes in vitro, Int. J. Dev. Biol.,41(1):83-9 (Feb. 1997) Abstract Only.
Iwasaki, S., et al.,Characterization of the bone morphogenetic protein-2 as a neurotrophic factor. Induction of neuronal differentiation of PC12 cells in the absence of mitogen-activated protein kinase activation, J. Biol. Chem.271(29):17360-5 (Jul. 1996) Abstract Only.
Kalyani, A.J., et al.,Spinal cord neuronal precursors generate multiple neuronal phenotypes in culture, J. Neurosci,18(19):7856-68 (Oct. 1998) Abstract Only.
Kitchener, P.D., et al.,Fetuin in neurons of the retina and cerebellum during fetal and postnatal development of the rat, Int. J. Dev. Neurosci,17(1):21-30 (Feb. 1999) Abstract Only.
Lee, Jacqueline E., et al.,Conversion of Xenopus Ectoderm into Neurons by NeuroD, a Basic Helix-Loop-Helix Protein, Science,vol. 268, pp. 836-844 (May 1995).
Lee, K.J., et al.,Neuronal patterning by BMPs: a requirement for GDF7 in the generation of a discrete class of commissural interneurons in the mouse spinal cord, Genes Dev.12(21):3394-407 (Nov. 1998) Abstract Only.
Lee, M.H., et al.,Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor betal in C2C12 myogenic cells coincides with suppression of the myogenic phenotype but is not sufficient for osteoblast differentiation, J. Cell Biochem.73(1):114-25 (Apr. 1999) Abstract Only.
Lein, P., et al.,Osteogenic protein-1 induces dendritic growth in rat sympathetic neurons, Neuron,15(3):597-605 (Sep. 1995) Abstract Only.
Ma, Qiufu, et al.,Identification of neurogenin, a Vertebrate Neuronal Determination Gene, Cell,vol. 87, 43-52 (Oct. 4, 1996).
Mayer-Proschel, M., et al.,Isolation of lineage-restricted neuronal precursors from multipotent neuroepithelial stem cells, Neuron,19(4):773-85 (Oct. 1997) Abstract Only.
McCormick, Mary B., et al.,neuroD2 and neuroD3: Distinct Expression Patterns and Transcriptional Activation Potentials within the neuroD Gene Family, Molecular and Cellular Biology,vol. 16, No. 10, p. 5792-5800 (Oct. 1996).
Merino, R., et al.,The BMP antagonist Gremlin regulates outgrowth, chondrogenesis and programmed cell death in the developing limb, Development,126(23):5515-5522 (1999) Abstract Only.
Murray, S.S., et al.,Bone morphogenetic protein inhibits differentiation and affects expression of helix-loop-helix regulatory molecules in myoblastic cells, J. Cell Biochem,53(1):51-60 (Sep. 1993) Abstract Only.
Nagata, T., et al.,Structure, backbone dynamics and interactions with RNA of the C-terminal RNA-binding domain of a mouse neural RNA-binding protein, Musashi 1, J. Mol. Biol.,287(2):315-30 (Mar. 1999) Abstract Only.
Nikaido, M. et al.,In vivo analysis using variants of zebrafish BMPR-IA: range of action and involvement of BMP in ectoderm patterning, Development,126(1):181-90 (Jan. 1999). Abstract Only.
Nakata, Katsunori, et al.,Xenopus Zic3, a primary regulator both in neural and neural crest development, Proc. Natl. Acad. Sci. USA,vol. 94, pp. 11980-11985 (Oct. 1997).
Park, J.K., et al.,Bipotent cortical progenitor cells process conflicting
Levesque Michel F.
Neuman Toomas
Cedars-Sinai Medical Center
Day Jones
McGarry Sean
Schultz J D
LandOfFree
Transdifferentiation of epidermal basal cells into neural... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Transdifferentiation of epidermal basal cells into neural..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Transdifferentiation of epidermal basal cells into neural... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3417498