Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1997-07-03
2000-07-11
McKelvey, Terry
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
435243, 4353201, 435410, 536 234, C12N 510, C12N 100, C12N 1562, C12N 1563
Patent
active
060871660
ABSTRACT:
Transcriptional activators which differ in their activation potential by more than 3 orders of magnitude are provided. The transactivators are fusions between a DNA binding protein (e.g., a Tet repressor) and minimal transcriptional activation domains derived from Herpes simplex virus protein 16 (VP16). Substitution mutations at amino acid position 442 within the minimal VP16 domain provide transactivators with differing transactivation ability. Moreover, chimeric activation domains comprising both wild type and mutant minimal VP16 domains provide additional variants with differing transactivation ability. Various aspects of the invention pertain to nucleic acid molecules, vectors, host cells, fusion proteins, transgenic and homologous recombinant organisms and methods of regulating gene transcription.
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Baron Udo
Bujard Hermann
Gossen Manfred
Baron Udo
BASF - Aktiengesellschaft
DeConti, Jr. Giulio A.
Kara Catherine J.
McKelvey Terry
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