Transcriptional activators

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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Details

C435S325000, C424S617000, C536S024100

Reexamination Certificate

active

06908761

ABSTRACT:
The invention provides a synthesis-inducible eucaryotic promoter having a transcriptional activity comprising, for example, a heavy metal-sensitive DNA sequence, derived from a natural promotor, of a base sequence of any one of SEQ ID NOS: 1 to 4; an inducer of the transcriptional activation activity thereof; a recombinant expression vector comprising a site for the insertion of an extraneous gene ligated to the above promotor: a eucaryotic expression kit comprising eucaryotic cells transfected with the above vector and the above inducer; and a method for expression of an extraneous gene in a eucaryotic system by using the same

REFERENCES:
patent: 4201775 (1980-05-01), Filan et al.
patent: 8-301875 (1996-11-01), None
Kataoka et al. Nukinzoku Ion Ni Yoru Mare Karen CiS-DNA Hairetsu No Tensha Kasseika, Dai 58kai,Nippon Gan Gakkai Soukai Kiji, Nippon Gan Gakkai, p. 483, column 1945 (1999), Applicant's IDS.
Matsushima-Hibiya et al. Rat Maf-related factors: the specificities of DNA binding and heterodimer formation Biochemical and biophysical Research Communications vol. 245, p412-418 1998.
Kobayashi et al,J. of Biol. Chem., 274(10):6443-6452 (1999).
Kataoka et al, “Jukinzoku Ion Ni Yoru Mare Karen Cis-DNA Hairetsu No Tensha Kasseika”, Dai 58kai, Nippon Gan Gakkai Soukai Kiji, Nippon Gan Gakkai, p. 483, column 1945 (1999).

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