Transcription template for cell-free protein synthesis and...

Details Transcription template for cell-free protein synthesis and... Transcription template for cell-free protein synthesis and...

2011-07-19

2011-07-19

Chunduru, Prabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024330

Reexamination Certificate

active

07981617

ABSTRACT:
Methods to construct a transcription template for cell-free protein synthesis that has a high translation template activity, using a 3′-side primer and a 5′-side primer for PCR are provided. The 5′-side primer for PCR has a sequence complementary to a base sequence containing at least a part of a promoter functional site from the 5′-end of a promoter and has a base sequence that does not contain a base sequence complementary to at least a part of a RNA polymerase-recognizing site of the 3′-end of the promoter. The other primer has a base sequence complementary to at least a part of the RNA polymerase-recognizing site of the 3′-end of the promoter and has a sequence that does not contain a base sequence complementary to at least a part of a promoter functional site from the 5′-end of the promoter.

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