Transcription method

Details Transcription method Transcription method

1995-11-22

1999-11-23

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 9121, 536 241, C12Q 168, C12P 1934, C07H 2102, C07H 2104

Patent

active

059898127

ABSTRACT:
An improved method of transcribing nucleic acids wherein the amount of abortive transcripts formed is reduced wherein the improvement comprises an effector sequence in the transcription reaction.

REFERENCES:
patent: 5474916 (1995-12-01), Reischl et al.
Sousa et al., "Model for the Mechanism of Bacteriophage T7 RNAP Transcription Initiation and Termination", J. Mol. Biol., 224: 319-334, (1992).
Promega 1993/1994 Catalog, p. 110, 1993/94.
Goeddel, D. (Ed) "Gene Expression Technology" Academic Press, Inc. pp. 512-527, 1991.

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