Transcription and nucleic acid sequence determination with short

Details Transcription and nucleic acid sequence determination with short Transcription and nucleic acid sequence determination with short

1993-01-14

1996-11-05

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 435 913, 435 915, 435 9151, 536 2433, 536 253, 536 254, 530350, 530820, 935 76, 935 77, C12Q 168, C12D 1934, C07H 2104, C12N 1500

Patent

active

055716695

ABSTRACT:
In vitro transcription of RNA or DNA templates from short RNA or DNA primers is acheived in the absence of a DNA promoter sequence. The progressive nature of the elongation complex according to the present invention allows for increased yields of fully extended transcripts and minimizes aborted RNA chains normally associated with in vitro transcription initiation at a promoter.

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