Transcript mapping method

Details Transcript mapping method Transcript mapping method

2011-08-23

2011-08-23

Zhou, Shubo (Department: 1631)

Data processing: measuring, calibrating, or testing

Measurement system in a specific environment

Biological or biochemical

C435S006120, C536S023100, C536S024100, C702S020000, C703S011000

Reexamination Certificate

active

08005621

ABSTRACT:
A transcript mapping method according to an embodiment of the invention is described hereinafter and combines short tag based (SAGE and MPSS) efficiency with the accuracy of full-length cDNA (flcDNA) for comprehensive characterization of transcriptomes. This method is also referred to as Gene Identification Signature (GIS) analysis. In this method, 5′ and 3′ terminal tags are obtained from a transcript and the terminal tags are for identifying 5′ and 3′ sites located on a genome sequence. Further, occurring segments and thus feasible gene locations are identified along the genome sequence after the 5′ and 3′ sites are identified. A data structure is also generated for indexing the genome sequence so that the terminal tags can be mapped to the genome sequence.

REFERENCES:
patent: WO 0238738 (2002-05-01), None
patent: WO 2004015085 (2004-02-01), None
“Data structure” definition from whatis.com.
“Bioinformatics” search from EAST.
Sadakane et al. Genome Informatics, vol. 12, pp. 175-183, 2001.
Wei et al. PNAS, vol. 101, pp. 11701-11706, Aug. 2004.

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