Trans-excision-splicing ribozyme and methods of use

Details Trans-excision-splicing ribozyme and methods of use Trans-excision-splicing ribozyme and methods of use

2007-05-01

2007-05-01

Schultz, James (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S320100, C435S006120

Reexamination Certificate

active

10730261

ABSTRACT:
A group I intron-derived ribozyme which binds RNA in trans, excises an internal segment from within the RNA, and splices the remaining 5′ and 3′ ends of the RNA back together (the trans-excision-splicing reaction) is disclosed. The excised segment can be as long as 28 nucleotides, or more, and as little as one nucleotide. The ribozymes of the invention are easily modified to alter their sequence specificity. Such ribozymes represent a new and potentially powerful class of generally adaptable genetic therapeutics.

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