Trans-dominant inhibition of geminiviral DNA replication by gemi

Details Trans-dominant inhibition of geminiviral DNA replication by gemi Trans-dominant inhibition of geminiviral DNA replication by gemi

1998-04-24

2000-09-12

McElwain, Elizabeth F.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide confers pathogen or pest resistance

435 691, 4353201, 435419, 435468, 536 2372, 800301, 800313, C12N 504, C12N 1533, C12N 1582, A01H 500, A01H 510

Patent

active

061180483

ABSTRACT:
A genetic construct comprising a trans-dominant inhibitor of geminiviral replication is disclosed. When expressed in a plant cell, this inhibitor is able to dramatically reduce replication of geminivirus. Genetic constructs that include sequences containing portion of the ac3 gene in addition to the trans-dominant inhibitor exhibit increased efficiency and broadened specificity of inhibition of geminiviral replication. A method of protecting plants against geminiviral infections is also disclosed.

REFERENCES:
Nejidat et al, Physiol. Plant., vol. 80, pp. 662-668, 1990.
Sijen et al, MPMI, vol. 8, pp. 340-347, 1995.
Koonin et al, J. Gen. Virol., vol. 73, pp. 2763-2766, 1992.
Russell et al, Plant Cell Rep., vol. 12, pp. 165-169, 1993.
Anderson et al, Proc. Natl. Acad. Sci., USA, vol. 89, pp. 8759-8763, 1992.
Hanson et al. "trans-Dominant Inhibition of Geminiviral DNA Replication by Bean Golden Mosaic Geminivirus rep Gene Mutants," Phytopathology 89:480-486.

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