Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-05-25
1988-04-19
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435235, 4351723, 435 91, 435320, 935 39, 935 34, 935 32, 536 27, C12N 1500, C12N 700, C12N 100
Patent
active
047389222
ABSTRACT:
This invention describes the discovery of a novel phenomena in retrovirus transcription, namely transcriptional trans-activation. Described herein are novel trans-acting factors which may be employed to enhance the production of heterologous genes. Described is a novel trans-acting directing gene, designated herein as the "luk" gene and the 35,000 to 45,000, more specifically about 42,000 dalton molecular weight protein encoded thereby.
The present invention demonstrates the LTR elements of HTLV can function as transcriptional promoters for heterologous genes on both unintegrated and integrated DNA. In general, the HTLV-1 LTR is a stronger promoter than is the HLTV-II LTR in its requirements for cellular and/or viral trans-acting factors in order to function efficiently. HTLV infection results in the production of trans-acting factors that dramatically increase the rate of HTLV LTR-promoted transcription.
REFERENCES:
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Green, M. et al., Nov. 1983, Cell 35:137-148.
Smith, G. et al., Dec. 1983, MCB 3:2156-2165.
Broome, S., 1982, In Eukaryotic Viral Vectors, pp. 139-144, (ed), Y. Gluzman, Cold Spring Harbor Laboratory, N.Y.
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Shimotohno, K. et al., Feb. 1984, PNAS 81:1079-1083.
Chen, I. et al., 17 May 1984, Nature 309:276-279.
Haseltine William A.
Rosen Craig A.
Sodrowski Joseph G.
Conlin David G.
Dana Farber Cancer Institute
Eisenstein Ronald I.
Linek Ernest V.
Seidman S.
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