Trains of yeast for the expression of heterologous genes

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351721, 435254, 435255, 435256, 4353201, 935 28, 935 37, 935 56, 935 61, 935 69, C12P 2102, C12N 1500, C12N 114, C12N 116, C12N 118, C12N 1581, C12N 119

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050681850

ABSTRACT:
The GAL4 protein is rate-limiting in quantity as a positive regulator for galactose-inducible promoters in strains of yeast. Novel strains are described in which the GAL4 protein can be overproduced in a regulatable fashion. These strains are useful for the regulatable expression in yeast of heterologous genes whose expression is driven by a galactose-inducible promoter.

REFERENCES:
Laughon et al., Mol. Cell Bio. vol. 4, pp. 268-275 (1984).

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