Toxin detection method

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

Reexamination Certificate

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C530S388100, C530S388200, C530S388400

Reexamination Certificate

active

07947808

ABSTRACT:
According to the present invention, an antibody against a Panton-Valentine leukocidin toxin contained inStaphylococcus aureus, a method and a kit for detecting the toxin with the use of the antibody, and a pharmaceutical composition containing an antibody against a Panton-Valentine leukocidin toxin for treating PVL infection caused byStaphylococcus aureuscontaining PVL are provided. Also, an antibody which is capable of binding to Panton-Valentine leukocidin F and has no cross-reactivity to LukD and/or HlgB and an antibody which is capable of binding to Panton-Valentine leukocidin S and has no cross-reactivity to at least one of LukE, HlgC, and HlgA are provided.

REFERENCES:
patent: 2008/0131457 (2008-06-01), Taylor et al.
patent: 2009/0074755 (2009-03-01), Taylor et al.
patent: 2000-316574 (2000-11-01), None
patent: WO 2006/078213 (2006-07-01), None
patent: WO 2006/135912 (2006-12-01), None
Gershon et al (Immunofluorescent Demonstration of Leucocidin Components pp. 477-495, 1968).
V. Finck-Barbancon et al., “Improved purification of leukocidin fromStaphylococcus aureusand toxin distribution among hospital strains”, Res. Microbiol. 1991, 142, 75-85.
Valerie Gauduchon et al., “Neutralization ofStaphylococcus aureusPanton Valentine Leukocidin by Intravenous Immunoglobulin in Vitro”, JID , Jan. 15, 2004:189 pp. 346-353.
A. Gravet D.A. et al., “Characterization of a novel structural member, LukE-LukD, of the bi-component staphylococcal leucotoxins family”, FEBS Letters, 436 (1998) 202-208.
G. Prevost et al., “Panton-Valentine Leucocidin and Gamma-Hemolysin fromStaphylococcus aureusATCC 49775 Are Encoded by Distinct Genetic Loci and Have Different Biological Activities”, Infection and Immunity, Oct. 1995, vol. 63, No. 10, pp. 4121-4129.
Wanna Choorit et al., “Existence of a new protein component with the same function as the LukF component of leukocidin or γ-hemolysin and its gene inStaphylococcus aureusP83”, FEBS Letters 357 (1995) 260-264.
Pascal Rainard et al., “Leucotoxic Activities ofStaphylococcus aureusStrains Isolated from Cows, Ewes, and Goats with Mastitis: Importance of LukM/LukF'-PV Leukotoxins”, Clinical and Diagnostic Laboratory Immunology, Mar. 2003, vol. 10, No. 2, pp. 272-277.
Supplementary European Search Report EP 07 76 8052 dated Jul. 14, 2010.
Kanenari Oishi et al., “A latex agglutination assay for specific detection of Panton-Valentine leukocidin”, Journal of Microbiological Methods, 75 (2008) pp. 411-415.
G. Prevost et al., “Epidemiological data onStaphylococcus aureusstrains producing synergohymenotropic toxins”, J. Med. Microbiol.—vol. 42 (1995) 237-245.

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