Toxicity screening method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

Reexamination Certificate

active

06998249

ABSTRACT:
The present invention provides materials and methods for predicting the in vivo toxicity of a given compound. For example, the invention comprises conducting at least three distinct assays in parallel to provide information about three distinct parameters of cytotoxicity of a chemical in a given target cell, which information is useful for predicting in vivo cytotoxicity.

REFERENCES:
patent: 4882270 (1989-11-01), Moroz
patent: 171085 (1994-06-01), None
patent: 2271772 (1994-04-01), None
patent: WO 95/32184 (1995-11-01), None
patent: WO 95/35278 (1995-12-01), None
patent: WO 97/49653 (1997-12-01), None
Renwick et al., Regulatory and Pharmacology 18: 463-480 (1993).
Eisenbrandt et al., Fd. Chem. Toxic. 32(7): 655-669 (1994).
Generaly & Applied Toxicology, vol. I, Stockton Press, New York, 1993, pp. 11-20.
Fricker, Toxic. In Vitro 8(4): 879-881 (1994).
Yao et al., Toxicology Methods 2(3): 199-218 (1992).
Chung et al., Chonnam J Med Sci 1(2): 128-138 (1988).
Morrison et al., Biomaterials 16(13): 987-992 (Sep. 1995).
Garza-Ocanas et al., Toxicology 73(2): 191-201 (1992).
Yao et al., Toxicology Methods (2):3: 199-218 (1992). Abstract.
Chung et al., Chonnam J Med Sci 1(2): 128-138 (1988). Abstract.
Morrison et al., Biomaterials 16(13): 987-992 (Sep. 1995). Abstract.
Garza-Ocanas et al., Toxicology 73(2): 191-201 (1992). Abstract.
Connors et al. (Biochem Pharmacol 24:2217-24), 1975.
Burkhardt, John E. et al., “Commentary: A View on Discovery Pathology,”Toxicologic Pathology,27(4):472-473 (1999).
Car, B.D. et al., “Commentary: Discovery Toxicology—A Nascent Science,”Toxicologic Pathology,27(4):481-483 (1999).
Cockerell, Gary L. et al., “Focus On: Discovery Pathology,”Toxicologic Pathology,27(4):471 (1999).
Cockerell, G.L. et al., “Commentary: Redesigning the Preclinical Paradigm: The Role of Pathology and Toxicology in Supporting Discovery Research,”Toxicologic Pathology,27(4):477-478 (1999).
Denizot, F. et al., “Rapid colorimetric assay for cell growth and survival Modifications to the tetrazolium dye procedure giving improved sensitivity and reliability,”J. Immunol. Methods.89:271-277 (1986).
Drug Discovery Using CombiChem'sDrug Discovery Engine™, http://www.combichem.com/Publication/Papers/Discovery.htm, pp. 1-6 (1997).
Essig-Marcello, J. et al., “A Double-LabelIn SituCytotoxicity Assay Using the Fluorescent Probes Neutral Red and BCECF-AM,” In vitrotoxicol,3(3):219-227 (1990).
Feinfeld, D.A. et al., “Urinary Glutathione-S-Transferase in Cisplatin Nephrotoxicity in the Rat,”J Clin Chem Clin Biochem.24:529-532 (1986).
Goegan, P. et al., “Effects of Serum Protein and Colloid on the AlamarBlue Assay in Cell Cultures,”Toxicol. In Vitro9(3):257-266 (1995).
Harleman, J.H., “Commentary Contribution of Pathology to Drug Research and Development in the Next Decade,”Toxicologic Pathology,27(4):479-480 (1999).
Kangas, L. et al., “Bioluminescence of Cellular ATP: A New Method For Evaluating Cytotoxic Agents In Vitro,”Med Biol,62:338-343 (1984).
Lowik, C.W.G.M. et al., “Quantification of Adherent and Nonadherent Cells Cultured in 96-Well Plates Using the Supravital Stain Neutral Red,”Anal. Biochem.213:426-433 (1993).
Mosmann, T., “Rapid Colorimetric Assay for Cellular Growth and Survival: Applicaiton to Proliferation and Cytotoxicity Assays,”J. Immunol. Methods,65:55-63 (1983).
Myers, P.L., “Re-enginering Of The Drug Discovery Process, Utilising A Unique Combination Of Library Design And Parallel Synthesis,” http://combichem.com/Publication/Papers/reenginering.htm, pp. 1-4 (1997).
Oberley, T. et al., “Effects of Lead Administration on Developing Rat Kidney II. Functional, Morphologic, and Immunohistochemical Studies,”Toxicol. Appl. Pharmacol.131:94-107 (1995).
Redick, J. et al., “Immunohistochemical Localization of Glutathionhe S-Transferases in Livers of Untreated Rats,”J. Biol. Chem,257:15200-15203.
Roehm, N. et al., “An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT,”J. Immunol. Methods,142:257-265 (1991).
Ryan, Anne M., “Commentary: Role of the Pathologist in the Identification and Characterization of Therapeutic Molecules,”Toxicologic Pathology,27(4):474-476 (1999).
The Universal Informer Library™: An Update, http://www.combichem.com/Publication/Papers/Train.htm, pp. 1-5 (1997).
Jung, et al., “Multiple Peptide Synthesis Methods and Their Applications”,angew Chem. Int. ed. Engl.,31:367-383 (1992).
Ellman and Bunin,J. Amer. Chem. Soc.,114:10997 (1992).
DeWitt, et al., “Diversomers”: An approach to nonpeptide, nonoligomeric chemical diversity,Proc. Nat'l. Acad. Sci.,90:6909 (1993).
Pavia, et al., “The Generation of Molecular Diversity”,Bioorg. Med. Chem. Lett.,3:387-396 (1993).
De Angelis, I. et al., “Established cell lines for safety assessment of food contaminants: differing furazolidone toxicity to V79, HEp-2, and CACO-2 cells,”Fd. Chem. Toxic.,32(5):481-488(1994).
Elmore, Eugene et al., “The human epithelial cell cytotoxicity assay for determining tissue specific toxicity,”Methods in Cell Science,22:17-24(2000).
Klostergaard, Jim et al., “Tomoricidal effector mechanisms of murineBacillus Calmette-Guerin-activated macrophages: mediation of cytolysis, mitochondrial respiration inhibition, and release of intracellular iron by distinct mechanisms,”Cancer Research,47:2014-2019(1987).
Steinbach O.M. and Wolterbeek H.Th., “Effects of copper on rat hepatoma HTC cells and primary cultured rat hepatocytes,”J. Inorg. Biochem.,53:27-48(1994).
Walum, E. et al., “Cellular methods for identification of neurotoxic chemicals and estimation of neurotoxicological risk,”Neurotoxicology,7(4):321-326(1993).
Wataha, J.C. et al., “The in vitro effects of metal cations on eukaryotic cell metabolism,”J. Biomed. Mater. Res.25:1133-1149(1991).
Jensen, M.T. et al., “Microbial production of skatole in the hind gut of pigs fed different diets and its relation to skatole deposition in backfat”, Proc of Meeting of EAAP Working Group, Meat & Livestock Comm., UK, Sep. 27-29, 1995.
Babol, J. et al., “Involvement of cytochrome P450IIE1 in hepatic metabolism and clearance of skatole”, EEAP Pub. No. 92, pp. 49-53. Oct. 1-3, 1997.
Babol, J. et al., “Relationship between oxidation and conjugation metabolism of skatole in pig liver and levels of skatole in fat”, EEAP Pub. No. 92, pp. 54-57. Oct. 1-3, 1997.
Laue, A. et al., “Effect of tryptophan infusion on the production of indole derivatives in the hind gut and absorption to the portal vein”, EEAP Pub. No. 92, pp. 58-61. Oct. 1-3, 1997.
Babol, J. et al., “Relationship between metabolism of androstenone and skatole”, EEAP Pub. No. 92, pp. 62-65. Oct. 1-3, 1997.
Edwards, S.M. et al., “Involvement of cytochrome b5 in androstenone biosynthesis”, EEAP Pub. No. 92, pp. 66-69. Oct. 1-3, 1997.
Andersson, K. et al., “Relations between boar taint and puberty in entire pig males”, EEAP Pub. No. 92, pp. 70-73. Oct. 1-3, 1997.
Lundström, K. et al., “Skatole levels in pigs selected for high lean tissue growth rate on different dietary protein levels”, Livestock Prod Sci, vol. 38, pp. 125-132, 1994.
Baek, C. et al., “Identificaiton of Selected Metabolites of Skatole in Plasma and Urine from Pigs”, J Agric Food Chem, vol. 45, pp. 2332-2340, 1997.
Babol, J, et al., “Relationship Between Oxidation and Conjugation Metabolism of Skatole in Pig Liver and Concentrations of Skatole in Fat”, J Anim Sci, vol. 76, pp. 829-838, 1998.
Babol, J. et al., “Hepatic Metabolism of Skatole in Pigs by Cytochrome P4502EI”, J Anim Sci, vol. 76, pp. 829-838, 1998.
Squires, E.J. et al., “Relationship Between Cytochr

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Toxicity screening method does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Toxicity screening method, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Toxicity screening method will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3669111

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.