Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-06
1999-09-07
Mertz, Prema
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 697, 435 71, 435471, 536 234, C12N 1509, C12N 1562
Patent
active
059486350
ABSTRACT:
A novel method for producing novel and/or improved heterofunctional binding fusion proteins termed Totally Synthetic Affinity Reagents (TSARs) is disclosed. TSARs are concatenated heterofunctional proteins, polypeptides or peptides comprising at least two functional regions: a binding domain with affinity for a ligand and a second effector peptide portion that is chemically or biologically active. In one embodiment, the heterofunctional proteins, polypeptides or peptides further comprise a linker peptide portion between the binding domain and the second active peptide portion. The linker peptide can be either susceptible or not susceptible to cleavage by enzymatic or chemical means. Novel and/or improved heterofunctional binding reagents as well as methods for using the reagents for a variety of in vitro and in vivo applications are also disclosed.
REFERENCES:
patent: 4522918 (1985-06-01), Schlom et al.
patent: 4612282 (1986-09-01), Schlom et al.
patent: 4732864 (1988-03-01), Tolman
patent: 4769326 (1988-09-01), Rutter
patent: 5096815 (1992-03-01), Ladner et al.
patent: 5162504 (1992-11-01), Horoszewicz
patent: 5198346 (1993-03-01), Ladner et al.
patent: 5223409 (1993-06-01), Ladner et al.
patent: 5270170 (1993-12-01), Cull et al.
patent: 5403484 (1995-04-01), Ladner et al.
Alting-Mees et al., "pBluescript II: Gene Mapping Vectors", 1989, Nucl. Acid Res. 17(22):9494.
Amann et al., "Tightly Regulated tac Promoter Vectors Useful for the Expression of Unfused and Fused Proteins in Escherichia coli", 1988, Gene 69:301-315.
Anand, et al., "Bacterial Expression and Secretion of Various Single-Chain Fv Genes Encoding Proteins Specific for a Salmonella Serotype B O-Antigen", 1991, J. Biol. Chem. 266(32):21874-21879.
Appel, J.R., "Elucidation of Discontinous Linear Determinants in Peptides", 1990, J. Immunol. 144(3):976-983.
Arkin and Youvan, "Optimizing Nucleotide Mixtures to Encode Specific Subsets of Amino Acids for Semi-Random Mutagenesis", 1992, Biotechnology 10:297-300.
Arnold, 1991, "Metal-Affinity Separations: A New Dimension in Protein Processing", Biotechnol. 9:151-156.
Baker et al., "A Gene Regulating the Heat Shock Response in Escherichia coli Also Affects Proteolysis", 1984, Proc. Natl. Acad. Sci. 81:6779-6783.
Baldwin et al., "Generation of a Catalytic Antibody by Site-Directed Mutgenesis", 1989, Science 245:1104-1107.
Barbas, et al., "Assembly of Combinatorial Antibody Libraries: A Chemical Solution to the Diversity Problem", 1992, proc. natl. Acad. Sci. USA 89:4457-4461.
Barbas, et al., Semisynthetic Combinatorial Antibody Libraries: A Chemical Solution to the Diversity Problem, 1992, Proc. Natl. Acad. Sci. USA 89:4457-4461.
Barbas, et al., "Human Monoclonal Fab Fragments Derived From a Combinatorial Library Bind to Respiratory Syncytial Virus F Glycoprotein and Neutralize Infectivity", 1992, Proc. Natl. Acad. Sci. 89:10164-10168.
Barnett et al., "Carcinoembryonic Antigen Family: Characterization of cDNAs Coding for NCA and CEA and Suggestion of Nonrandom Sequence Variation in Their Conserved Loop-Domains", 1988, Genomics 3:59-66.
Barrett, et al., "Selective Enrichment and Characterization of High Affinity Ligands from Collections of Random Peptides on Filamentous Phage", 1992, Anal. Biochem. 204:357-364.
Bass, et al., "Hormone Phage: An Enrichment Method for Variant Proteins With Altered Binding Properties", 1990, Proteins 8:309-314.
Beaudry and Joyce, "Directed Evolution of an RNA Enzyme", 1992, Science 257:635-641.
Berg, "Potential Metal-Binding Domains in Nucleic Acid Binding Proteins", 1986, Science 232:485-487.
Berg, "Proposed Structure for the Zinc-Binding Domains from Transcription Factor IIIA and Related Proteins", 1988, Proc. Nat'l Acad. Sci. USA 85:99-102.
Berg, "Zinc Finger Domains: Hypotheses and Current Knowledge", 1990, Ann. Rev. Biophy. Biochem. 19:405-421.
Birnbaum and Mosbach, "Peptide Screening", 1992, Current Opinion in Biotechnology 3:49-54.
Blondelle, et al., "Evaluation of Peptide-Peptide Interactions Using Reversed-Phased High-Performance Liquid Chromatography", 1992, J. Chromatography 625:199-206.
Cabilly et al., "Generation of Antibody Activity From Immunoglobulin Polypeptide Chains Produced in Escherichia coli", 1984, Proc. Natl. Acad. Sci 81:3273-3277.
Cesareni, G., "Peptide Display on Filamentous Phage Capsids--A New Powerful Tool to Study Protein-Ligand Interaction", 1992, FEBS Letters 307(1):66-70.
Chakrabarrty et al., "Large Differences in the Helix Propensities of Alanine and Glycine", 1991, Nature 351:586-588.
Chapman, et al., 1992, Biochemistry 31:12819-25.
Charbit, et al., "Summary--Expression of Foreign Peptides by Genetic Fusion in Bacteria and Bacteriophages", 1971, Bulletin de L Institut Pasteur 89(1):17-49 (Abstract only).
Charbit et al., "Versatility of a Vector for Expressing Foreign Polypeptides at the Surface of Gram-Negative Bacteria", 1988, Gene 70: 181-189.
Charbit et al., "Permissive Sites and Topology of an Outer Membrane Protein with a Reporter Epitope", 1991, J. Bacteriol. 173(1):262-275.
Chaudhary et al., "A Rapid Method of Cloning Functional Variable-Region Antibody Genes in Escherichia coli as Single-Chain Immunotoxins", 1990, Proc. Natl. Acad. Sci. 87:1066-1070.
Cherny et al., "cDNA Sequence, Protein Structure, and Chromosomal Location of the Human Gene for Poly(ADP-ribose) Polymerase", 1987, Proc. Natl. Acad. Sci. 84:8370-8374.
Chien, et al., "Diagnosis of Hepatitis C Virus (HCV) Infection Using an Immunodominant Chimeric Polyprotein to Capture Circulating Antibodies: Reevaluation of the Role of HCV in Liver Disease", 1992, Proc. Natl. Acad. Sci. USA 89:10011-10015.
Christian, et al., "Simplified Methods for Construction, Assessment and Rapid Screening of Peptide Libraries in Bacteriophage", 1992, J. Mol. Biol. 227:711-718.
Clackson, et al., "Making Antibody Fragments Using Phage Display Libraries", 1991, Nature 352:624-628.
Clement and Hofnung, "Gene Sequence of the .lambda. Receptor, an Outer Membrane Protein of E. coli K12", 1981, Cell 27:507-514.
Clement et al., "Bacterial Vectors to Target and/or Purify Polypeptides: Their Use in Immunological Studies", 1991, Ann. Bio. Clin. 49:249-254.
Clement et al., "Molecular and Cellular Targeting in the Expression of Foreign Polypeptides in Bacteria", 1992, Antonie van Leeuwenhoek 61:143-152.
Collet, et al., "A Binary Plasmid System for Shuffling Combinatorial Antibody Libraries", Nov. 1992, Proc. Natl. Acad. Sci. 89:10026-10030.
Crissman and Smith, "Gene-III Protein of Filamentous Phages: Evidence for a Carboxyl-Terminal Domain with a Role in Morphogenesis", 1984, Virology 132:445-455.
Cull et al., "Screening for Receptor Ligands Using Large Libraries of Peptides Linked to the C Terminus of the lac Repressor", 1992, Proc. Natl. Acad. Sci. USA 89:1865-1869.
Cwirla, et al., "Peptides on Phage: A Vast Library of Peptides for Identifying Ligands", 1990, Proc. Natl. Acad. Sci. USA 87:6378-6382.
De la Cruz, et al., "Immunogenicity and Epitope Mapping of Foreign Sequences Via Genetically Engineered Filamentous Phage", 1988, J. Biol. Chem. 263:4318-4322.
Derbyshire et al., "A Simple and Efficient Procedure for Saturation Mutagenesis Using Mixed Oligodeoxynucleotides", 1986, Gene 46:145-152.
Devlin, et al., "Random Peptide Libraries: A Source of Specific Protein Binding Molecules", 1990, Science 249:404-406.
Dower, et al., "The Search for Molecular Diversity (II): Recombinant and Synthetic Randomized Peptide Libraries", 1991, Ann. Rep. Med. Chem. 26:271-279.
Dunn et al., 1988, Protein Eng. 2:283-291.
Evan et al., "Isolation of Monoclonal Antibodies Specific for Hnuman c-myc Prote-Oncogene Product", 1985, Mol. Cell. Biol. 5:3610-3616.
Evans, "The Steroid and Thyroid Hormone Receptor Superfamily", 1988, Nature 240:889-895.
Feild, et al., "Structure-Function Analysis of Human Transforming Growth Factor-.alpha. by Site-Directed Mutagenesis", 1992, J. Biochem. 283:91-98.
Felici, F., "Selection of Antibody Ligands from a Large Library of Oligopeptides Expressed on a Multivalent Exposition Vector", 1991, J. Mol. Biol. 222:301-310.
Fields et al., "A Novel Genetic System to Detect Prot
Adey Nils B.
Fowlkes Dana M.
Kay Brian K
Sparks Andrew B.
Mertz Prema
University of North Carolina at Chapel Hill
LandOfFree
Totally Synthetic Affinity Reagents does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Totally Synthetic Affinity Reagents, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Totally Synthetic Affinity Reagents will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1803768