Total protein detection method

Details Total protein detection method Total protein detection method

1997-09-23

1999-06-01

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436169, G01N 3348

Patent

active

059087870

ABSTRACT:
Disclosed is an improved assay for the determination of total protein in an aqueous test fluid such as urine. The assay involves combining the test fluid with a molybdate or tungstate salt and a dye which forms a complex with molybdate or tungstate ion at a pH of 1.0 to 3.0 to shift the absorption band of the complex in the presence of protein. There is introduced into the assay a substituted phenolsulfonephthalein dye having a pKa which enables it to operate at a pH of from about 1.0 to 3.0 and whose affinity for protein is such that it will provide a detectable response only in the presence of greater than 50 mg/dL of protein to increase the scope of the assay.

REFERENCES:
patent: 4960710 (1990-10-01), Lau
patent: 5055407 (1991-10-01), Lau et al.
patent: 5087575 (1992-02-01), Lau
patent: 5399498 (1995-03-01), Pugia

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