Total cysteine assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

Reexamination Certificate

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C435S004000, C435S975000

Reexamination Certificate

active

06927038

ABSTRACT:
A method to determine a total cysteine in biological fluids utilizes similarly treated portions of the fluid with a homocysteinase and a non-specific desulfurase.

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patent: 6265220 (2001-07-01), Ullman
patent: 6468762 (2002-10-01), Tan
patent: 2002/0123088 (2002-09-01), Matsuyama et al.
patent: 2000-270895 (2000-10-01), None
patent: 2000-338096 (2000-12-01), None
patent: WO 98/007872 (1998-02-01), None
patent: WO 01/077670 (2001-10-01), None
English Language Translation of JP 2000-270895A (Ebinuma et al.) Oct. 3, 3000, Japanese Version provided by Applican Machine Translation provided by Japanese Patent Office Web Site, Oct. 5, 2004, pp. 1-8.
Han et al., Protein Expression and Purification (1998) 14(2):267-274.
Supplementary European Search Report for EP 02 78 4538, mailed on Nov. 15, 2004, 3 pages.
Tanaka et al., Agricultural and Biological Chemistry (1981) 45(4):1021-1022.
Han et al., Protein Expression & Purification (1998) 14:267-274.
Tan et al., Protein Expression & Purification (1997) 9:233-245.
El-Khairy et al., “Plasma Total Cysteine as a Risk Factor for Vascular Disease. The European Concerted Action Project” Circulation 103:2544-2549 (2001).
Horton et al., “Relationships Among Plasma Homocysteine, Cysteine, and Albumin Concentrations: Potential Utility of Assessing the Cysteine Homocysteine Ratio” J. Clin. Chem. 47:1121-1124 (2001).
Mansoor et al., “Redox Status and Protein Binding of Plasma Aminothiols During the Transient Hyperhomocysteinemia That Follows Homocysteine Administration” Clin. Chem. 39:980-985 (1993).

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