Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2007-12-25
2007-12-25
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
C435S069100, C435S252300
Reexamination Certificate
active
11447367
ABSTRACT:
Compositions and methods are provided for the enhanced in vitro synthesis of protein molecules, by optimizing the metabolism of amino acids present in the reaction mix, preferably all amino acids in the reaction mixture. By performing synthesis with extracts from genetically modified microbial strains that are deficient in multiple amino acid metabolizing enzymes reduces the enzymatic activities responsible for catalyzing these reactions and improves the overall yield of synthesis.
REFERENCES:
patent: 6168931 (2001-01-01), Swartz et al.
patent: 6337191 (2002-01-01), Swartz et al.
patent: 6559176 (2003-05-01), Bassler et al.
patent: 2002/0081660 (2002-06-01), Swartz et al.
patent: 2004/0209321 (2004-10-01), Swartz et al.
patent: WO 00/55353 (2000-09-01), None
patent: WO 2004/016778 (2004-02-01), None
patent: WO 2005/010155 (2005-02-01), None
Metabolic Engineering, Issue 3, Jul. 2004, pp. 197-203; Available on line Apr. 27, 2004.
Datsenko, K., et al., “One-step inactivation of chromosomal genes inEscherichia coliK-12 using PCR products,” (2000)PNAS, 97(12):6640-6645.
Kim, D., et al., “A highly efficient cell-free protein synthesis fromEscherichia coli,” (1996)Eur. J. Biochem, 239:881-886.
Su, et al., “L-serine degradation inEscherichia coliK-12: Cloning and sequencing ofsdaAgene,” (1989)Journal of Bacteriology, 171(9):5095-5102.
Jewett, M., et al., “Mimicking theEscherichia colicytoplasmic environment activates long-lived and efficient cell-free protein synthesis,” (2004)Published online by Wiley InterScience(www.interscience.wiley.com, DOI 10/1002/bit.20026.
Kudlicki, W., et al., “High efficiency cell-free synthesis of proteins: refinement of the coupled transcription/translation system,” (1992)Analytical Biochemistry, 206:389-393.
Michel-Reydellet, N., et al., “Amino acid stabilization for cell-free protein synthesis by modification of theEscherichia coligenome,” (2004) 6:197-203.
Pratt, J., “Coupled transcription-translation in prokaryotic cell-free systems,” (1984)Transcription and translation: a practical approach, Hames, B. and Higgins, S., eds., pp. 179-209.
Jewett, M., et al., “Prokaryotic systems for in vitro expression,” (2002)Gene cloning and expression technologies, Weiner, M. and Lu, Q., eds., pp. 391-411.
Shao, et al., “Sequencing and characterization of thesdaBgene fromEscherichia coliK-12,” 91993)Eur. J. Biochem. 212:777-784.
Calhoun Kara Anne
Swartz James Robert
Bozicevic Field & Francis LLP
Saidha Tekchand
Sherwood Pamela J.
The Board of Trustees of the Leland Stanford Junior University
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