Agitating – By injecting gas into mixing chamber
Reexamination Certificate
2009-07-16
2010-12-14
Cooley, Charles E (Department: 1774)
Agitating
By injecting gas into mixing chamber
C261S077000, C435S296100
Reexamination Certificate
active
07850365
ABSTRACT:
A device for circulating a liquid media in a vessel includes a gas infusion tube having a leading end immersed in the liquid media and a trailing end in fluid communication with a remote air pump. A diffuser is secured to the leading end of the gas infusion tube. The lumen of an outer tube receives the gas infusion tube and the diffuser. The outer tube has a leading end disposed in spaced relation to a bottom wall of the vessel and has a trailing end substantially flush with a level of the liquid media. A plurality of circumferentially spaced openings are formed in the trailing end of the outer tube or in a cap secured to the trailing end. Bubbles are emitted from the diffuser, entraining the liquid media so that it follows a toroidal path of travel that maximizes cell growth.
REFERENCES:
patent: 2744065 (1956-05-01), Lacey
patent: 4957623 (1990-09-01), Henzlik
patent: 5190647 (1993-03-01), Balestrieri
patent: 7363878 (2008-04-01), McRobert
patent: 2009/0303829 (2009-12-01), Muecke
Treece, D., et al., Laboratory Manual for the Culture of Penaeid Shrimp Larvae, TAMU-SG, 1988, vol. 88, No. 202, pp. 35-47.
Much Alan Maxwell
Much, legal representative Carolyn
Cooley Charles E
Smith Ronald E.
Smith & Hopen , P.A.
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