Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Conjugate or complex
Patent
1982-02-23
1984-08-07
Rosen, Sam
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Conjugate or complex
424 95, A61K 3574
Patent
active
044643624
DESCRIPTION:
BRIEF SUMMARY
It is known that excessive exposure to solar radiation can cause chronic skin damage, which can lead to epidermal changes, such as atrophy, pigment displacements, collagenous degeneration up to permanent damage of the desoxyribonucleic acid (DNA) of the cells. The sequence of the elements of the double-stranded helix structure of the DNA, as is known, during cell division secures the correct replication of the genetically coded hereditary dispositions. Intensive exposure of the skin to UV radiation leads to a thymidine dimerisation by cycloaddition between two adjacent thymidine elements of the DNA. This change in the structure of the nucleic acid can lead to either the death of the cell or to inheritable cell damage.
Each cell, however, possesses a so-called endogenous repair system, consisting of an enzymatically controlled reaction sequence in order to cut the thymidine dimers out of the DNA strand and to replace these by thymidine monomers.
This natural DNA repair mechanism, above all, possesses only a limited capacity which is extensively exhausted by an excessive intense effect of light and sun. Moreover, it is known from investigations, (J. B. Little ("Gerontology" 22, 28-55 (1976)), that between the aging of a cell and its ability to permit the course of a normal repair process, there exists a close relationship wherein it still must be explained as to whether a reduction in the repair ability of the cell is the direct cause for the aging or it represents only an accelerating factor.
Accordingly, it is an object of the present invention to provide cosmetic compositions, the use of which allows to increase the body's own DNA repair capacity of the skin cells, reduced by excessive influence of light to the physiologically required level and in this manner preclude permanent cell damage and retard the light-caused aging process of the skin.
The foregoing object is achieved by local application of cosmetic compositions to the skin, the compositions containing an active substance complex which promotes the DNA repair process of the skin cells. Ascertained as being particularly suitable therefore is an active substance obtained from inactivated cultures of bacteria of the genus Bifidobacterium or related types which is capable of causing a desirable increase in the body's own repair capacity for the skin cells, the repair capacity of which has been reduced by the influence of light.
By the application of these cosmetic compositions permanent cell damage can be prophylactically counteracted and the light-caused aging process of the skin retarded, which imparts to these compositions a high practical value from the cosmetic viewpoint.
The inventive active substance complex which promotes the repair process consists, for example, of inactivated bacteria of the genus Bifidobacterium, such as the species Bifidobacterium longum (Reuter). These contain the cytoplasm fraction with enzymes, such as lactic acid dehydrogenase, phosphatases, phosphoketolases and transaldolases, and in addition murein and polysaccharides.
The inactivated cells, for example, can be obtained by rinsing anaerobically obtained surface cultures of bifido bacteria with physiological NaCl solution, centrifugation, resuspending in physiological NaCl solution and treating with ultrasound (20 KHz) whilst cooling in an ice-bath to 0.degree. C. until all viable organisms are killed. For this a period of about 20 minutes is required for each 100 ml of the solution. For the inactivation of the cells there are also other known mechanical operating methods that can be used, e.g., J. R. Norris and D. D. Ribbons "Methods in Microbiology" Volume 5B, pages 1 though 54 (1971) Publishers: Academic Press, London and New York.
However, there can be also utilized as the starting material bacteria related to the genus Bifidobacterium, such as are listed, for example, in Bergery's Manual of Determinative Bacteriology, 8th Edition (1975), under Actinomycetaceae, Propionibacteriaceae, Lactobacillaceae and coryneform bacteria (refer to, among others, pages 576, 59
REFERENCES:
patent: 4057627 (1977-11-01), Stickl
Winter, A Consumer's Dictionary of Cosmetic Ingredients, Crown Pub., N.Y., 1976, pp. 51, 159, 160.
Clark, Secrets of Health & Beauty, Jove/HBJ, N.Y. .COPYRGT.1969, 2nd Jove/HBJ printing Feb., 1979, pp. 16, 17, 104, 105, 158, 159.
Komeiji, Chem. Abs., vol. 87, 1977, Ab. No. 87: 141124b.
Yamashita, Chem. Abs., vol. 88, 1978, Ab. No. 88: 110537e.
Bergey's Manual of Determ. Bacti., Williams & Wilkins, Baltimore, Md., 8th Ed., 1974, pp. 576, 599, 633, 659, 660.
Davis et al., Microbiol., Harper-Row, Hagerstown, Md., 2nd Ed., 1973, pp. 783-802.
Frobisher et al., Fundamentals of Microbiol., W. B. Saunders, Phila., Pa., 9th Ed., 1974, pp. 72-85, 94, 95, 572-575, 577, 578, 580-582, 585, 586, 588.
Heise Dieter
Kludas Martin
Estee Lauder Inc.
Rosen Sam
LandOfFree
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