Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2011-04-19
2011-04-19
Bui, Phuong T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C435S006120, C435S183000, C435S411000, C530S370000, C536S023200, C536S023600, C800S260000
Reexamination Certificate
active
07928298
ABSTRACT:
A series of independent non-transgenic mutations found in the fruit PG gene of tomato; tomato plants having these mutations in their fruit PG gene; and a method of creating and identifying similar and/or additional mutations in the PG gene by screening pooled and/or individual tomato plants. The tomato plants of the present invention exhibit reduced PG enzyme activity and fruit that soften more slowly post harvest without having the inclusion of foreign nucleic acids in their genomes.
REFERENCES:
patent: 4801540 (1989-01-01), Hiatt et al.
patent: 5107065 (1992-04-01), Shewmaker et al.
patent: 5387747 (1995-02-01), Bru-Magniez et al.
patent: 5413937 (1995-05-01), Bridges et al.
patent: 5442052 (1995-08-01), Bird et al.
patent: 5447867 (1995-09-01), Bridges et al.
patent: 5453566 (1995-09-01), Shewmaker et al.
patent: 5569831 (1996-10-01), DellaPenna
patent: 5759829 (1998-06-01), Shewmaker et al.
patent: 5994075 (1999-11-01), Goodfellow
patent: WO-00/63347 (2000-10-01), None
patent: WO-01/75167 (2001-10-01), None
Ali et al. (1982). “Purification and Characterization of the Polygalacturonases of Tomato Fruits,”Aust. J. Plant Physiol. vol. 9, pp. 155-169.
Anthon at al. (2002). “Thermal Inactivation of Pectin Methylesterase, Polygalacturonase, and Peroxidase in Tomato Juice,”Journal of Agricultural and Food Chemistry, vol. 50, pp. 6153-6159.
Atkinson et al. (1993). “A Polygalacturonase Gene From Kiwifruit (Actinidia deliciosa),”Plant Physiology103:669-670.
Bird et al. (1988). “The Tomato Polygalacturonase Gene and Ripening-Specific Expression in Transgenic Plants,”Plant Mol. Biol., vol. 11, pp. 651-662.
Brummell, D.A. et al. (Sep. 2001). “Cell Wall Metabolism in Fruit Softening and Quality and its Manipulation in Transgenic Plants,”Plant Molecular Biology47(1/2):311-340.
Cantwell, M. (Jan. 8, 2002). “Report to the California Tomato Commission: Tomato Variety Trials: Postharvest Evaluations for 2001.”
Chen et al. (1999). “A Rapid DNA Minipreparation Method Suitable for AFLP and Other PCR Applications,”Plant Molecular Biology Reporter, vol. 17, pp. 53-57.
Colbert et al. (Jun. 2001). “High-Throughput Screening for Induced Point Mutations,”Plant Physiology, vol. 126, pp. 480-484.
Cooley et al. (1996). “Site-Selected Insertional Mutagenesis Of Tomato With Maize Ac And Ds Elements,”Mol. Gen. Genet., vol. 252(1-2), pp. 184-194.
Cooley et al. “Insertional Inactivation of the Tomatoe Polygalaturonase Gene,”J. I., Plant Mol. Biol., vol. 38(4) pp. 521-530, 1998.
Della Penna et al. (1986). “Molecular Cloning of Tomato Fruit Polygalacturonase: Analysis of Polygalacturonase mRNA Levels During Ripening,”Proc. Natl. Acad. Sci. U.S.A., vol. 83, pp. 6420-6424.
Edan, Y. (1997). “Color and Firmness Classification of Fresh Market Tomatoes,” Journal of Food Science, vol. 62(4) pp. 793-796.
Eriksson, E.M. et al. (Dec. 2004). “Effect of the Colorless Non-Ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening,”Plant Physiology136:4184-4197.
Errington, N. (1997). “Changes in the force relaxation and compression response of tomatoes during ripening: The Effect of Continual Testing and Polygalacturonase Activity,”Postharvest Biology and Technology, vol. 11, pp. 141-147.
Fachin et al. (2002). “Thermal and High-Pressure Inactivation of Tomato Polygalacturonase: A Kinetic Study,”Journal of Food Science, vol. 67, pp. 1610-1615.
GenBank Accession No. M37304, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=170472> visited on Aug. 14, 2007. (4 pages).
GenBank Accession No. X07410, located at <http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=19295> visited on Sep. 26, 2007. (2 pages).
Grierson, D. (Nov. 11, 1986). “cDNA Clone For Tomato Polygalacturonase,”Nucleic Acids Res., vol. 14(21), pp. 8595-8603.
Grierson, D. et al. (1989). “Physiological and Genetic Factors Governing the Expression of the Polygalacturonase Gene in Ripening Tomatoes,”UCLA Symposia on Molecular and Cellular Biology92:169-179.
Guo et al. (2004). “Protein Tolerance to Random Amino Acid Change,”PNAS101:9205-9210.
Hamilton, A.J. (1995). “Sense and Antisense Inactivation Of Fruit Ripening Genes In Tomato,”Current Topics in Microbiol Immunol, vol. 197, pp. 77-89.
Henikoff et al. (2000). “Increased Coverage of Protein Families With the Blocks Database Servers,”Nucl. Acids Res. vol. 28, pp. 228-230.
Henikoff et al. (1999). “Blocks+: A Non-Redundant Database Of Protein Alignment Blocks Derived From Multiple Compilations,”Bioinformaticsvol. 15(6), pp. 471-479.
Henikoff et al. (1995). “Automated Construction and Graphical Presentation Of Protein Blocks From Unaligned Sequences,”Gene, ISSN 0378-1119 Amsterdam, Elsevier, vol. 163, pp. GC17-GC26.
Hill et al. (1998). “Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase FromEscherichia coli,” Biochemical and Biophysical Research Communications244:573-577.
Hobson, G.E. et al. (1994). “From Producer to Pantry-using Biotechnology to Preserve Crop Quality,”Aspects of Applied Biology39:95-102.
Innis, M. A. et al. eds. (1990).PCR Protocols: A Guide to Methods and Applications. Academic Press Inc., pp. 3-21.
Jarret, R.L. et al. (1984). “Ripening Behavior of the Green Ripe Tomato Mutant,”J. Amer. Soc. Hort. Sci. 109(5):712-717.
Ju R. et al. (1994). “Cloning of Polygalacturonase (PG) Cdna and Inhibition Effects Of Its Antisense RNA On The Expression of PG Gene In Transgenic Tomato Plants,”Chin. J. Biotechnol., vol. 10(2), pp. 67-74.
Kalaitzis et al. (1997). “Three Different Polygalacturonases Are Expressed In Tomatoe Leaf And Flower Abscission, Each With A Different Temporal Expression Pattern,”Plant Physiology, vol. 113, pp. 1303-1308.
Knapp, J. et al. (Jan. 1989). “Organization and Expression of Polygalacturonase and other Ripening Related Genes in Ailsa Craig ‘Neverrip’ and ‘Ripening Inhibitor’ tomato mutants,”Plant Molecule Biology12(1):105-116.
Kramer et al. (1992). “Postharvest Evaluation Of Transgenic Tomatoes With Reduced Levels Of Polygalacturonase: Processing, Firmness And Disease Resistance,”Postharvest Biology and Technology, vol. 1, pp. 241-255.
Lazar et al. (1988). “Transforming Growth Factor: Mutation of Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities,”Molecular and Cellular Biology8:1247-1252.
Lesage et al. (1996). “Measurement of Tomato Firmness by Using a Non-Destructive Mechanical Sensor,”Postharvest Biology and Technology, vol. 8, pp. 45-55.
Li et al. (May 2002). “Integrated Platform For Detection of DNA Sequence Variants Using Capillary Array Electrophoresis,”Electrophoresis, vol. 23(10), pp. 1499-1511.
McCallum et al. (Apr. 2000). “Targeted Screening for Induced Mutations,”Nature Biotechnology, vol. 18, pp. 455-457.
McCallum et al. (Jun. 2000). “Targeting Induced Local Lesions IN Genomes (Tilling) For Plant Functional Genomics,”Plant Physiology, vol. 123, pp. 439-442.
McCallum, C. M. et al. (Jul. 2003). “Tilling for Loss of Function Alleles in the Tomato Fruit Specific Polygalacturonase,” American Society of Plant Biologists: Plant Biology 2003, Honolulu, Hawaii, 4 pages (Table of Contents).
Murray, A.J. et al. (1995). “Evaluation of Transgenic Tomato Fruit with Reduced Polygalacturonase Activity in Combination with the rin Mutation,”PostHarvest Biology and Technology6:91-101.
Neff et al. (1998). “dCAPS, a Simple Technique for the Genetic Analysis of Single Nucleotide Polymorphisms: Experimental Applications In Arabidopsis Thaliana Genetics,”The Plant Journal, vol. 14, pp. 387-392.
Oleykowski
Colbert Trenton G.
Hurst Susan
Knauf Vic C.
McCallum Claire
Slade Ann J.
Arcadia Biosciences Inc.
Bui Phuong T
Morrison & Foerster / LLP
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