Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Reexamination Certificate
2006-10-20
2010-11-02
Kruse, David H (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
C383S127000
Reexamination Certificate
active
07825305
ABSTRACT:
The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generateNicotiana tobacumplant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improvedNicotiana tobacumplant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.
REFERENCES:
patent: 3722516 (1973-03-01), Suwa et al.
patent: 4407307 (1983-10-01), Gaisch et al.
patent: 4537204 (1985-08-01), Gaisch et al.
patent: 5539093 (1996-07-01), Fitzmaurice et al.
patent: 5668295 (1997-09-01), Wahab et al.
patent: 5670349 (1997-09-01), Cramer et al.
patent: 5705624 (1998-01-01), Fitzmaurice et al.
patent: 5741898 (1998-04-01), Hanley et al.
patent: 5837876 (1998-11-01), Conkling et al.
patent: 6030462 (2000-02-01), Shu et al.
patent: 6730832 (2004-05-01), Dominguez et al.
patent: 7173170 (2007-02-01), Liu et al.
patent: WO 02/100199 (2002-12-01), None
Williams et al. 1968. Tobacco Science 128: 243-247.
Imsande. 2001. Crop Sci. 41: 510-515.
Currin et al. 1981. Crop Sci. 21: 988.
Bright, S. W. J. et al., “Two Genes for Threonine Accumulation in Barley Seeds,” Nature, 299: 278-279 (1982).
Bright, S. W. J. et al., “Threonine Accumulation in the Seeds of a Barley Mutant with an Altered Aspartate Kinase,” Biochem. Genet., 20: 229-243 (1982).
Broun, P. et al., “Progress in Plant Metabolic Engineering,” PNAS, 98: 8925-8927 (2001).
Cattoir-Reynaerts, A. et al., “Selection and Characterization of Carrot Embryoid Cultures Resistant to Inhibition by Lysine Plus Threonine,” Biochem. Physiol. Pflanzen, 178: 81-90 (1983).
Chen, J. et al., “Comparison of Volatile Generation in Serine/Threonine/Glutamine-Ribose/Glucose/Fructose Model Systems,” J. Agric. Food Chem., 47: 643-647 (1999).
Diedrick, T. J. et al., “Tissue Culture Isolation of a Second Mutant Locus for Increased Threonine Accumulation in Maize,” Theor. Appl. Genet., 79: 209-215 (1990).
Dotson, S. B. et al., “Lysine-Insensitive Aspartate Kinase in Two Threonine-Overproducing Mutants of Maize,” Planta, 182: 546-552 (1990).
Ellstrand, N. C., “When Transgenes Wander, Should We Worry?,” Plant Physiol., 125: 1543-1545 (2001).
Falco, S. C. et al., “Using Bacterial Genes to Engineer Plants with Increased Seed Lysine,” SIM News, 47(2): 53-57 (1997).
Falco, S. C. et al., “Transgenic Canola and Soybean Seeds with Increased Lysine,” Biotechnology, 13: 577-582.
Fehr, W. R., “Mutation Breeding,” in Applied Plant Breeding, Chapter 6, pp. 6-1 to 6-30, 2nded. Iowa State University, Ames, IA (1983).
Frankard, V. et al., “Two Feedback-Insensitive Enzymes of the Aspartate Pathway inNicotiana sylvestris,” Plant Physiol., 99: 1285-1293 (1992).
Frankard, V. et al., “High Threonine Producer Mutant ofNicotiana sylvestris(Spegg. And Comes),” Theor. Appl. Genet., 82: 273-282 (1991).
Galili, G. et al., “Enhancing the Content of the Essential Amino Acids Lysine and Threonine in Plants,” in: Plant Amino Acids in Biochemistry and Biotechnology, B. K. Singh, ed., Marcel Dekker, Inc., New York, pp. 487-507 (1999).
Galili, G., “Regulation of Lysine and Threonine Synthesis,” The Plant Cell, 7: 899-906 (1995).
Haughn, G. et al., “Selection for Herbicide Resistance at the Whole Plant Level,” in: Biotechnology in Agricultural Chemistry, H. M. Lebaron (ed.), American Chemical Society, Washington, D.C., pp. 98-107 (1987).
Heremans, B. et al., “Threonine Accumulation in a Mutant ofArabidopsis thaliana(L.) Heynh, with an Altered Aspartate Kinase,” J. Plant Physiol., 146: 249-257 (1995).
Hibberd, K. A. et al., “Selection and Characterization of a Feedback-Insensitive Tissue Culture of Maize,” Planta, 148: 183-187 (1980).
Karchi, H. et al., “Seed-Specific Expression of a Bacterial Desensitized Aspartate Kinase Increases the Production of Seed Threonine and Methionine in Transgenic Tobacco,” The Plant Journal 3: 721-727 (1993).
Lea, P. et al., “The Biosynthesis of Amino Acids in Plants” in: Chapter 6, Chemistry and Biochemistry of the Amino Acids, G. C. Barrett, (ed.), Chapman & Hill, London, pp. 197-226 (1985).
Lu, G. et al., “Generation of Flavor Compounds by the Reaction of 2-Deoxyglucose With Selected Amino Acids,” J. Agric. Food Chem., 45: 233-236 (1997).
Malmberg, R. et al., “Chapter 2; Production and Analysis of Plant Mutants, EmphasizingArabidopsis thaliana,” in: Methods in Plant Molecular Biology and Biotechnology, B. R. Glick and J.E. Thompson, (eds.), CRC press (1993).
Matthews, B. F., “Lysine, Threonine and Methionine Biosynthesis,” in: Plant Amino Acids Biochemistry and Biotechnology, B. K. Singh, (ed.), Marcel Dekker, Inc., New York, pp. 205-225 (1999).
Peng, J. et al., “Derivative Alleles of the Arabidopsis Gibberellin-Insensitive (gai) Mutation Confer a Wild-Type Phenotype,” The Plant Cell, 5: 351-360 (1993).
Shaul, O. et al., “Threonine Overproduction in Transgenic Tobacco Plants Expressing a Mutant Desensitized Aspartate Kinase ofEscherichia coli,” Plant Physiol., 100: 1157-1163 (1992).
Williams, J. et al., “Changes in Amino Acid Content of Flue-Cured Tobacco During Natural Aging,” Tobacco Science, 128: 243-247 (1968).
Van Harten, A. M., in: Mutation Breeding, Theory and Practical Applications, Chapters 1 and 2, pp. 1-63, Cambridge University Press (1998).
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Dominguez Luis Mayan
Dube Michael Francis
Lawson Jerry Wayne
Liu Wennuan
Kilpatrick & Stockton LLP
Kruse David H
Reynolds Technologies, Inc.
Robinson Keith O.
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