TNF-inhibitory protein and a method of production

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

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530351, 530369, C07K 1500

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active

051360217

ABSTRACT:
A TNF-inhibitory protein having a molecular weight of 28 kilodaltons and having an activity of rendering resistant to TNF a TNF- sensitive cell to thereby inhibit the cytolytic and/or cytostatic activity of TNF for the TNF-sensitive cells and a method for producing the same are described. The TNF-inhibitory protein can advantageously be used as an antigen for producing an antibody useful for detecting a patient having TNF-resistant cancer and for removing the TNF-resistance of the cancer cells of the patient.

REFERENCES:
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Kamijo et al. Biochem. Biophys. Res. Comm. 158(1) pp. 155-162 (1989).
Purification and initial characterization of a type .beta.transforming growth factor from human placenta Charles A. Frolik et al. Proc. Natl. Acad. Sci. USA, 80: 3676-3680, 1983.
A Tumor Necrosis Factor-binding Protein Purified to Homogeneity from Human Urine, Protects Cells from Tumor Necrosis Factor Toxicity/Hartmut Engelmann et al. J. Biol. Chem. 264: 11974-11980, 1989.
Purification and Biologic Characterization of a Specific Tumor Necrosis Factor a Inhibitor Philippe Seckinger et al. J. Bio. Chem., 264: 11966-11973, 1989.
A Tumor Necrosis Factor Binding Protein is present in human biological fluids Christina Peetre et al., Eur. J. Haem., 41: 414-419, 1988.
Philippe Seckinger et al. J. Biol. Chem. 264: 11966-11973, 1989.
A Tumor Necrosis Factor Binding Protein is present in human biological fluids, Christina Peetre, et al. Eur. J. Haem., 41:414-419, 1988.

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