TMB Formulation for soluble and precipitable HRP-ELISA

Details TMB Formulation for soluble and precipitable HRP-ELISA TMB Formulation for soluble and precipitable HRP-ELISA

1990-01-26

1991-05-07

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435967, 435962, C12Q 100, G01N 3353

Patent

active

050136461

ABSTRACT:
Solvent systems for use in increasing the solubility of 3,3',5,5'-tetramethylbenzidine (TMB) and for making a permanent color record of the results of ELISAs using HRP have been developed. In the improved solvent system containing povidone, 1-ethenyl-2-pyrrolidinone polymers, TMB can be dissolved in a very small quantity of solvent, up to a concentration of 100 to 200 NM, which can then be mixed directly into an aqueous buffer without precipitating the TMB. In the solvent system for making a permanent record, alginic acid (AA), methyl vinyl ether/maleic anhydride copolymer (MVE/MAC), dextran sulfate (DS) and/or carrageenan are added to the aqueous buffer solution, with or without povidone, to form a colored precipitate with the reaction product.

REFERENCES:
patent: 4615972 (1986-10-01), Gallacher
J. L. Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, Jan. 7, 1987, p. 1238.

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