TM4 conditional shuttle phasmids and uses thereof

Details TM4 conditional shuttle phasmids and uses thereof TM4 conditional shuttle phasmids and uses thereof

1997-09-26

1999-10-26

Ketter, James

Chemistry: molecular biology and microbiology

Vector, per se

435440, 435471, 435473, 435474, C12N 1563

Patent

active

059727004

ABSTRACT:
The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the TM4 mycobacteriophage that introduces DNA of interest into mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a TM4 conditional shuttle phasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and a method of generating auxotrophic mutants.

REFERENCES:
patent: 5504005 (1996-04-01), Bloom et al.
Seres et al. Zbl. Bakt. vol. 275, pp. 54-62, 1991.
MsAdam et al. Infection and Immunity, vol. 63, No. 3, pp. 1004-1012, Mar. 1995.
Kleckner et al. in Methods in Enzymology, vol. 204, pp. 139-142 and 157-160, 1991.

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