Tissue processing for immunofluorescence microscopy

Details Tissue processing for immunofluorescence microscopy Tissue processing for immunofluorescence microscopy

1982-11-22

1990-11-20

Brown, Johnnie R.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

435 1, G01N 100, G01N 130, A01N 100

Patent

active

049717839

ABSTRACT:
A tissue holding and processing technique suitable for immunofluorescence, histochemical, and light microscopic analysis of the same specimen. The tissue is first placed in formol sucrose, and then transferred to gum sucrose. The tissue is subsequently washed in phosphate buffered saline, then acetone-water, then acetone, and finally in xylene. Finally, the tissue is embedded in paraffin. Tissue processed according to the present invention may be stored indefinitely in the gum sucrose or paraffin.

REFERENCES:
patent: 2393580 (1946-01-01), Weiskopf
patent: 3961097 (1976-06-01), Gravlee, Jr.
Holt, S. J. et al. J. Biophys. Biochem. Cytol vol. 7, 1960 p. 383-386.
Holt, S. J. et al. J. Biophys. Biochem. Cytol. vol. 11, 1961 p. 31-45.
Humason, G. L., Animal tissue Techniques, W. H. Freeman & Co., San Francisco 1972, p. 35,419.
Culling, C. F. A., Handbook of Histopathological and Histochemical Techniques, Butterworths, London, 1974, p. 46,629.

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