Tissue preservation method with...

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...

Reexamination Certificate

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Reexamination Certificate

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07824847

ABSTRACT:
Tissue preservation media comprising a polyoxyethylene/polyoxypropylene copolymer are used to preserve tissues and organs for storage and transplantation. In particular embodiments, the polyoxyethylene/polyoxypropylene copolymer is Pluronic F68 or FLOCOR (CRL-5861; purified poloxamer 188), and the medium is Steinhardt medium, polyoxyethylene/polyoxypropylene copolymer-supplemented Optisol GS or polyoxyethylene/polyoxypropylene copolymer-supplemented ViaSpan.

REFERENCES:
patent: 7087369 (2006-08-01), Steinhardt
patent: 7371513 (2008-05-01), Steinhardt
patent: 2009/0017438 (2009-01-01), Roy et al.
patent: WO 2004/099393 (2004-11-01), None
Solberg et al., “A new protective solution for hypothermic storage of free vein grafts in cardiovascular surgery”, Scand. J. Clin. Lab. Invest. 52: 73-82 (1992).
Moghimi et al., “Causative factors behind poloxamer 188 (Pluronic F68, Flocor ™)-induced complement activation in human sera. . . ”, Biochimica et Biophysica Acta 1689: 103-113 (2004).
Santoli et al., “Effects of storage with University of Wisconsin solution on human saphenous vein endothelium”, J. Thorac. Cardiovasc. Surg. 108: 382-383 (1994).
Lowe KC et al: “Beneficial Effects of Pluronic F-68 and Artificial Oxygen Carriers on the Post-Thaw Recovery of Cryopreserved Plant Cells” Artificial Cells, Blood Substitutes, and Immobilization Biotechnology, Marcel Dekker Inc, US, vol. 29, No. 4, Jul. 1, 2001, pp. 297-316, XP009071457.
Steven J. Meier et al.: “Cell death from bursting bubbles: Role of cell attachment to rising bubbles in sparged reactors” Biotechnology and Bioengineering, vol. 62, No. 4, Feb. 20, 1999 ( ), pp. 468-478, XP007911641.
Anonymous: “Ex-Cell 420 Frequently Asked Questions” Internet Citation Jan. 2003, XP002396013 Retrieved from the Internet: URL:http://web.archive.org/web/20050221073 332/http://www.jrhbio.com/Document.aspx?ID=319> [retrieved on Oct. 15, 2004J Y 9th question; p. 2, col. 1.
Voss Wa et al: “Combined and Individual Protection by Pluronic Polyols and Dimethyl Sulfoxide to Human Erythrocytes Recovered From Liquid Nitrogen” Cryobiology, Academic Press Inc, US, vol. 11, No. 4, Aug. 1, 1974 pp. 285-295, XP009071445.
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