Tissue plasminogen activator variants with decreased clearance

Details Tissue plasminogen activator variants with decreased clearance Tissue plasminogen activator variants with decreased clearance

1993-03-16

1994-08-16

Hill, Jr., Robert J.

Drug, bio-affecting and body treating compositions

Enzyme or coenzyme containing

Hydrolases

424 9463, 435212, 435226, C12N 964, C12N 1500, A61K 37547

Patent

active

053385462

ABSTRACT:
Biologically active tissue plasminogen activator (t-PA) variants with decreased clearance as compared to wild-type t-PA are prepared, including variants that have one or more amino acid alterations in at least the kringle-1 and/or kringle-2 domain(s) of the molecule. DNA sequences can be prepared that encode the variants, as well as expression vectors incorporating the DNA sequences and host cells transformed with the expression vectors. The variants may be used in pharmaceutical preparations to treat a vascular disease or condition, or to prevent fibrin deposition or adhesion formation or reformation in mammals.

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