Tissue culture technique utilizing a specific light source

Details Tissue culture technique utilizing a specific light source Tissue culture technique utilizing a specific light source

1976-12-20

1977-12-06

Bagwill, Robert E.

Plant husbandry

Process

47DIG6, A01G 100

Patent

active

040609332

ABSTRACT:
One aspect of the invention comprises a method for stimulating organogenesis of explants in a tissue culture comprising the step of illuminating the explant culture during at least a portion of its differentiation stage with light having a predominate spectral emission of approximately 660nm.
A second aspect of the invention includes the additional illumination of the explants with 740nm light, subsequent to an intervening period of preferably one to two weeks. The 660nm light primarily stimulates shoot formation while the 740nm light primarily stimulates root formation. The intervening period precludes photoreversibility of the initially obtained light effects.

REFERENCES:
patent: 3514900 (1970-06-01), McDade
patent: 3683550 (1972-08-01), Corlett et al.
patent: 3816960 (1974-06-01), Gudin et al.

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