Tissue culture system

Details Tissue culture system Tissue culture system

1978-09-21

1980-06-17

Penland, R. B.

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

435313, C12M 300

Patent

active

042084830

ABSTRACT:
A tissue culture system for zero gravity high-density cultivation of eucaryotic cells requiring attachment to a substratum in order to differentiate is disclosed. A major component of the system is a cylindrical bottle filled with culture medium and having mounted therein a rotatable shaft. The shaft includes a plurality of collagen-treated discs projecting therefrom and at an angle to the shaft to provide the necessary attachment substratum and to impel the culture medium as the shaft is rotated. Means are provided for initial stocking of the bottle with the culture cells and for continuously supplying fresh medium to and removing waste medium from the bottle.

REFERENCES:
patent: 3028314 (1962-04-01), Means et al.
patent: 3062724 (1962-11-01), Reusser
patent: 3839155 (1974-10-01), McAleer et al.
patent: 4004981 (1977-01-01), Hurni et al.
patent: 4065359 (1977-12-01), Hurni

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