Tissue culture process for producing a large number of viable mi

Details Tissue culture process for producing a large number of viable mi Tissue culture process for producing a large number of viable mi

1997-01-31

1999-04-27

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Plant cell or cell line, per se ; composition thereof;...

Culture, maintenance, or preservation techniques, per se

435431, C12N 500

Patent

active

058980016

ABSTRACT:
The present invention relates to a tissue culture process for producing a large number of viable mint plants in vitro. The process of the present invention employs specified pieces of an internodal segment of the stem of the mint plant as the starting material and identifies medium and culture conditions for producing a large number of plants. Such plants can be used for micropropagation, selection of mutants, production of plants with altered levels of endogenous secondary metabolites and for genetic engineering.

REFERENCES:
Kukreja et al., "Screening and evaluation of agronomically useful somaclonal variations in Japanese mint, Mentha-arvensis L.", Euphytica 53 (3) : 183-192 (1991).
Bhat et al., "Plant regeneration from various explants of cultivated Paper species", Plant Cell Reports 14 : 398-402 (1995).
Lubomski et al., "In vitro propagation of pot carnation from stem internodes", Acta Horticulturae 251 : 235-240 (1989).
C. Berry, J.M. Van Eck, S.L. Kitto and A. Smigocki, "Agrobacterium-mediated transformation of commercial mints", Plant Cell, Tissue and Organ Culture 44:177-181 (1996).
J.-C. Caissard, O. Faure, F. Jullien, M. Colson, and A. Perrin, "Direct regeneration in vitro and transient GUS expression in Mentha x piperita", Plant Cell Reports 16:67-70 (1996).
G.D. Coleman and S.G. Ernst, "In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype", Plant Cell Reports 8:459-462 (1989).
A.K. Kukreja, O.P. Dhawan, A.K. Mathur, P.S. Ahuja, and S. Mandal, "Screening and evaluation of agronically useful somaclonal variations in Japanese mint (Mentha arvensis L.)", Euphytica 53(3):183-191 (1991).
A.K. Kukreja, O.P. Dhawan, P.S. Ahuja, S. Sharma, and A.K. Mathur, "Genetic improvement of mints: On the qualitative traits of essential oil of in-vitro derived clones of Japanese mint (Mentha arvensis var. piperascens Holmes)", J. Essent. Oil Res. 4:623-629 (1992).
A. Spencer, J.D. Hamill, and M.J.C. Rhodes, "Production of terpenes by differentiated shoot cultures of Mentha citrata transformed with Agrobacterium tumefaciens T37", Plant Cell Reports 8:601-604 (1990).

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