Tissue culture method for asexual propagation of pine trees and

Plant husbandry – Process

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043543278

ABSTRACT:
A tissue culture method for the asexual propagation of pine trees is described and claimed. The method involves promoting adventitious buds from pine seeds by inoculating decoated seeds that have been previously stimulated to germinate in a culture medium. The method enhances adventitious bud formation and is operationally more practical than currently reported methods in that it is neither time consuming nor labor intensive. Also, novel culture media are described and claimed which employ the interaction of the growth regulators benzylaminopurine and abscisic acid.

REFERENCES:
patent: 4038778 (1977-08-01), Kadkade
patent: 4152869 (1979-05-01), Jones
Stimulation of adventitious . . . , Heide, Nature, 219, 1968, pp. 960-961.
Interaction of abscisic acid . . . , Basu et al., Plant and Cell Physiol., 47, 1971, pp. 681-684.
Differention of plantlets . . . , Sommer et al., Bot. Gaz., 136, 1970, pp. 196-200.
Promotion of callus . . . , Altman et al., Plant Physiol., 47, 1971, 844-846.
Development and differentiation . . . , Gresshof et al., Planta (Berlin), 107, 1972, pp. 161-170.
The effects of abscisic . . . , Ammirato, Bot. Gaz., 135 (4), 1974, pp. 328-337.
Induction of multiple buds . . . , Campbell et al., Can. J. Bot., 53, 1975, pp. 1652-1657.
Adventitious bud formation . . . , Cheng, Plant Sci. Letters, 5, 1975, pp. 97-102.
Vegetative propagation . . . , Campbell et al., Can. J. For. Res., 6, 1976, pp. 240-243.
Production of forest trees . . . , Mott et al., TAPPI, 60, No. 6, 1977.
Shoots from Douglas-Fir cultures, Can. J. Bot., 55, No. 9, 1977, pp. 1246-1250.
Hormonal Control of . . . , Mehra-Palta et al., TAPPI, 61, No. 1, 1978.

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