Tissue culture medium

Details Tissue culture medium Tissue culture medium

1983-01-26

1985-08-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, 424101, 260112B, C12N 500, C12N 502, A61K 3514, A23J 106

Patent

active

045336342

ABSTRACT:
A growth factor containing serum is disclosed which is derived from natural bovine serum is disclosed which is by precipitating a first solids fraction from natural bovine serum by adding ammonium sulfate to about 25% saturation to give a first solids fraction and a first supernatant; separating the first solids fraction from the first supernatant; precipitating a second solids fractions from the first supernatant by adding ammonium sulfate to about 40% saturation to give a second solids fraction and a second supernatant; separating the second solids fraction from the second supernatant; combining the first solids fraction and the second supernatant; and desalinating the first solids fraction and the second supernatant, either before or after combination thereof.

REFERENCES:
patent: 3122476 (1964-02-01), Gaeta
patent: 3128228 (1964-04-01), Michl
patent: 3429867 (1969-02-01), Bozicevich
patent: 3686395 (1972-08-01), Stephan
patent: 4038139 (1977-07-01), Birch
patent: 4059512 (1977-11-01), Harris
Fisher et al., Biochemistry, vol. 44, pp. 4-10, 1958.
Puck et al., PNAS, vol. 59, pp. 192-199, 1968.
Ham et al., in Methods in Enzymology, vol. 58, pp. 77-93, 1979.
Temin et al., in Growth Nutrition and Metabolism of Cells in Culture, vol. I, Academic Press, NY., pp. 49-81, 1972.
Hayashi, I. et al., Nature, vol. 259, No. 5539, pp. 132-134, 1976.
Parker, Methods in Tissue Culture, Harper & Row, NY, p. 193, 1961.

Affiliated with

Also associated with

Rating

Tissue culture medium does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Tissue culture medium, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Tissue culture medium will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-512624