Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1980-04-04
1982-03-23
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435298, 435299, 435300, 435284, C12M 120, C12M 300
Patent
active
043213308
ABSTRACT:
A disposable tissue culture device, particularly for aerobic cultures, comprising a tissue culture dish containing at least one shallow culture chamber for the growth of microorganisms, and a cover for the culture dish to prevent the entrance of contaminants, the cover being in contact with the contents of each culture chamber over the entire culture chamber to provide optimal viewing of each culture chamber by light microscopy through a transparent cover window, standoffs being provided between the cover and the culture dish to assure atmospheric communication between each culture chamber and ambient atmosphere, minimum annular space being provided between the cover and upper edge of each culture chamber to maintain an almost enclosed, constant volume in each culture chamber to hold the culture stable during culturing, handling and vibration, yet permit the culture to breathe. The transparent cover window portion of the cover enclosing the contents of each culture chamber is substantially flat, and has a slight convex, upward curve on the inside surface adjacent to the edge to permit egress of bubbles due to de-gassing of fluid in each culture chamber. An annular fluid reservoir, provided around the upper edge of each culture chamber, permits compensation for evaporation of fluid from the culture and provides a tolerance on the filling volume. Lift lugs are provided on opposite sides of the dish extending outward beyond the cover and upward to facilitate moving the culture dish without disturbing the cover located thereon, the lift lugs extending only partway around the dish to permit easy removal and replacement of the cover for administration of specimens and other ingredients to each culture chamber.
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Baker Fraser L.
Baumann John H.
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