Tissue biopsy cell suspender for cell analysis

Details Tissue biopsy cell suspender for cell analysis Tissue biopsy cell suspender for cell analysis

1996-09-24

1997-09-16

Redding, David A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 4051, 435 4052, 4352876, 435810, C12Q 102, C12M 300

Patent

active

056679858

ABSTRACT:
A tissue biopsy cell suspender device includes a receptacle having a sealable opening. The receptacle contains a sufficient quantity of an aqueous medium to support an enzymatic dissociation of cells from a tissue biopsy sample. The device has a removable closure with a first sealing position and a second sealing position for sealing the opening in the receptacle. The device further includes a frangible container having a sufficient quantity of an enzyme therein to dissociate cells from the tissue biopsy sample. The frangible container is covered with a membrane that is permeable to water and to the enzyme. The frangible container is disposed within the receptacle so that the container is intact when the closure is in the first sealing position and the container is breached when the closure is in the second sealing position. When the frangible container is breached, the enzyme is released to permeate into the medium and for dissociation of the cells from the tissue sample.

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patent: 5073488 (1991-12-01), Matner et al.
"Cell Isolation Theory," Worthing Manual Tissue Dissociation Guide, Pp. 2-14.
"Culture Of Animal Cells," by R. Ian Freshney, Wiley-Liss, pp. 116-125.
"Cell Culture," by William B. Jakoby and Ira H. Pastan, Methods in Enzymology, pp. 119-130.
"Worthing Enzyme Manual, enzymes, enzyme reagents, related biochemicals," Worthington Biochemical Corporation, (1972), pp. 125-127.
"Neuropathology Of AIDS In Surgical Biopsy Specimens," by Douglas C. Miller, MD, PhD, Souhel Najjar, MD and Gleb N. Budzilovich, MD, Neurosurgery Clinics of North America, vol. 5, No. 1, Jan. 1994, pp. 57-70 .

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