Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving cholesterol
Patent
1989-01-03
1991-09-10
Rosen, Sam
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving cholesterol
435 19, 435 28, C12Q 160
Patent
active
050473271
ABSTRACT:
There is provided a stable cholesterol assay composition which comprises an aqueous solution of at least one bile acid or salt thereof being present in an amount of up to about 5 mM; a nonionic surfactant present in an amount of from about 0.15 to about 1.5 percent volume by volume; a buffer in a concentration of from 0 to about 65 mM; and cholesterol oxidase in a concentration of at least about 0.1 kIU/l. Solution pH is from about 5.5 to about 8.5. Addition of cholesterol esterase, phenol, peroxidase and 4-aminoantipyrine provides a total cholesterol chromogen system.
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Allain, C. C. et al., "Enzymatic Determination of Total Serum Cholesterol", Clinical Chem., vol. 20, No. 4, pp. 470-475 (1974).
Caris Karen R.
Modrovich Ivan E.
Modrovich Ivan E.
Rosen Sam
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