Time-resolved fluorimetric detection of lanthanide labeled nucle

Chemistry: analytical and immunological testing – Biospecific ligand binding assay

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436536, 436546, 436800, 436 94, 436 56, G01N 33566

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active

049620452

ABSTRACT:
Nucleotides conjugated with a chelating agent and labeled with a lanthanide, especially terbium, are readily detected by time-resolved fluorimetry during and following gel electrophoresis. The labeled nucleotide conjugates are very stable, highly fluorescent with a long radiative lifetime and remain fluorescent during dilution and during gel electrophoresis and since an enhancement solution is not required for detection, the labeled nucleotide conjugates can be detected on-line, if desired.

REFERENCES:
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Dechaud et al., "Laser Excited Immunofluorometric Assay of Prolactin with Use of Antibodies Coupled to Lanthanide-Labeled DTPAA", (Abstract only) Clin. Chem. 32(7), 1986 1323-1327.
Syvanen, A. C. et al., Nucl. Acids Res. 14, 1017-1028 (1986).
Bailey, N. P. et al., Analyst, 109, 1449-1450 (1984).
Bailey, N. P. et al, Analyst, 110, 603-604 (1985).
Weider, I., Background Rejection in Fluorescence Immunoassay, Immunofluorescence and Related Staining Techniques, 67-80 (1978).
Dahlen, P. Anal. Biochem., 164. 78-83 (1987).

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